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Literature summary for 2.3.1.85 extracted from
- Characterization of the polyspecific transferase of murine type I fatty acid synthase (FAS) and implications for polyketide synthase (PKS) engineering (2018), ACS Chem. Biol., 13, 723-732 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | expression of a didomain subconstruct KS-MAT (2?853), bearing a beto-ketoacyl synthase KS knockout C161G and malonyl/acetyltransferase MAT | Mus musculus |
| R606A | active site mutant of didomain construct. The specificity constant for malonyl-CoA is 40fold smaller than the wild-type constant | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00062 | - |
methylmalonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus |  |
| 0.00073 | - |
octanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 0.00086 | - |
acetoacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 0.00096 | - |
phenylacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 0.00128 | - |
malonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 0.00136 | - |
crotonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 0.00163 | - |
acetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 0.00164 | - |
butanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 0.00172 | - |
3-hydroxybutanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 0.00206 | - |
3-methylbutanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 0.0021 | - |
succinyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 0.003 | - |
acetyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
| 0.0048 | - |
palmitoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 0.018 | - |
malonyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | P19096 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxybutanoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + 3-hydroxybutanoyl-[acyl-carrier protein] | - |
? | |
| 3-methylbutanoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + 3-methylbutanoyl-[acyl-carrier protein] | - |
? | |
| acetoacetyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + acetoacetyl-[acyl-carrier protein] | - |
? | |
| acetyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + acetyl-[acyl-carrier protein] | - |
? | |
| butanoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + butanoyl-[acyl-carrier protein] | - |
? | |
| crotonyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + crotonyl-[acyl-carrier protein] | - |
? | |
| malonyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + a malonyl-[acyl-carrier protein] | - |
? | |
| methylmalonyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + a methylmalonyl-[acyl-carrier protein] | - |
? | |
| octanoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + octanoyl-[acyl-carrier protein] | - |
? | |
| palmitoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + palmitoyl-[acyl-carrier protein] | - |
? | |
| phenylacetyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + phenylacetyl-[acyl-carrier protein] | - |
? | |
| succinyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + succinyl-[acyl-carrier protein] | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
palmitoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 4 | - |
methylmalonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 4.1 | - |
octanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 5 | - |
malonyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
| 6 | - |
crotonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 6 | - |
phenylacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 6.1 | - |
3-hydroxybutanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 7.2 | - |
3-methylbutanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 7.3 | - |
acetoacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 10.8 | - |
butanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 13 | - |
acetyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
| 13.4 | - |
succinyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 13.7 | - |
acetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 15.6 | - |
malonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 300 | - |
malonyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
| 500 | - |
palmitoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 4000 | - |
acetyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
| 4400 | - |
crotonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 5600 | - |
octanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 6200 | - |
phenylacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 6400 | - |
succinyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 6500 | - |
methylmalonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 6600 | - |
butanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 8400 | - |
acetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 8500 | - |
acetoacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
| 12200 | - |
malonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
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