reconstitution of fatty acid synthase using purified protein components. When all ketosynthases are present at 1 microM, the maximum rate of free fatty acid synthesis of the fatty acid synthase exceeds 100 microM/min. The steady-state turnover frequency is not significantly inhibited at high concentrations of any substrate or cofactor. Fatty acid synthase activity is saturated with respect to most individual protein components when their concentrations exceeded 1 microM. The exceptions are protein components FabI and FabZ, which increase fatty acid synthase activity up to concentrations of 10 microM, FabH and FabF, which decrease fatty acid synthase activity at concentrations higher than 1 microM, and holo-acyl carrier protein and TesA, which give maximum fatty acid synthase activity at 30 microM concentrations |
Escherichia coli |