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Literature summary for 2.3.1.85 extracted from

  • Muesing, R.A.; Porter, J.W.
    Fatty acid synthase from pigeon liver (1975), Methods Enzymol., 35, 45-49.
    View publication on PubMed
Search for more literature for 2.3.1.85

General Stability

General Stability Organism
dithiothreitol stabilizes Columba sp.
foaming leads to surface inactivation Columba sp.
low ionic strength leads to dissociation of native enzyme and inactivation Columba sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-values for various partial activities of fatty acid synthetase Columba sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Gallus gallus
-
-
soluble
-
 Rattus norvegicus
-
-
soluble
-
 Columba sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
450000
-
-
 Columba sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ Gallus gallus multifunctional enzyme, involved in animal fat synthesis palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ Rattus norvegicus multifunctional enzyme, involved in animal fat synthesis palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ Columba sp. multifunctional enzyme, involved in animal fat synthesis palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Columba sp.
-
 pigeon
-
Gallus gallus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Gallus gallus
homogeneity Rattus norvegicus
homogeneity Columba sp.

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O reaction mechanism Gallus gallus
a
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O reaction mechanism Rattus norvegicus
a
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O reaction mechanism Columba sp.
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-
liver
-
 Rattus norvegicus
-
liver
-
 Columba sp.
-
mammary gland
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.078 0.1 palmitate Columba sp.
1.12 1.4 NADPH, 30°C Columba sp.

Storage Stability

Storage Stability Organism
-20°C, at least a month in the presence of DTT Columba sp.
-20°C, under nitrogen at least 1-2 months Columba sp.
4°C, 90% loss of activity in 8 days with 40% dissociation of native enzyme, incubation at 38°C with 10 mM DTT restores activity Columba sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
-
 Gallus gallus palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O in the absence of NADPH the product is triacetic acid lactone ?
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
-
 Rattus norvegicus palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O in the absence of NADPH the product is triacetic acid lactone ?
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ specific for malonyl-CoA, acetyl-CoA can be replaced by propionyl-CoA or butyryl-CoA Columba sp. palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O in the absence of NADPH the product is triacetic acid lactone ?
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ multifunctional enzyme, involved in animal fat synthesis Gallus gallus palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ multifunctional enzyme, involved in animal fat synthesis Rattus norvegicus palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ multifunctional enzyme, involved in animal fat synthesis Columba sp. palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Gallus gallus
25
-
 assay at Rattus norvegicus
25
-
 assay at Columba sp.
30
-
 assay at Gallus gallus
30
-
 assay at Rattus norvegicus
30
-
 assay at Columba sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 50% loss of activity in dilute, mildly alkaline solution within 3.5 h, high ionic strength together with DTT reactivates Columba sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Gallus gallus
6.8
-
 assay at Rattus norvegicus
6.8
-
 assay at Columba sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.4
-
 inactivation, decreased by 0.2 M KCl or 0.02 mM NADP(H) Columba sp.

Cofactor

Cofactor Comment Organism Structure
4'-phosphopantetheine
-
 Gallus gallus
4'-phosphopantetheine
-
 Rattus norvegicus
4'-phosphopantetheine
-
 Columba sp.
additional information no requirement for FMN Gallus gallus
additional information no requirement for FMN Rattus norvegicus
additional information no requirement for FMN Columba sp.
NADH requirement, 10% as effective as NADPH Columba sp.
NADPH
-
 Gallus gallus
NADPH
-
 Rattus norvegicus
NADPH
-
 Columba sp.