BRENDA - Enzyme Database show
show all sequences of 2.3.1.84

The purification, properties and internal peptide sequences of alcohol acetyltransferase isolated from Saccharomyces cerevisiae Kyokai No. 7

Minetoki, T.; Bogaki, T.; Iwamatsu, A.; Fujii, T.; Hamachi, M.; Biosci. Biotechnol. Biochem. 57, 2094-2098 (1993)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
complete inhibition
Saccharomyces cerevisiae
Cd2+
-
Saccharomyces cerevisiae
Cu2+
-
Saccharomyces cerevisiae
Hg2+
-
Saccharomyces cerevisiae
linoleic acid
2 mM, 86% inhibition
Saccharomyces cerevisiae
linolenic acid
2 mM, 68% inhibition
Saccharomyces cerevisiae
p-chloromercuribenzoate
complete inhibition
Saccharomyces cerevisiae
Zn2+
-
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
acetyl-CoA
strain NCYC 366
Saccharomyces cerevisiae
0.19
-
acetyl-CoA
-
Saccharomyces cerevisiae
29.8
-
isoamyl alcohol
-
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
x * 22000, SDS-PAGE
Cladosporium cladosporioides
60000
-
x * 60000, SDS-PAGE
Saccharomyces cerevisiae
270000
-
gel filtration
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cladosporium cladosporioides
-
-
-
Saccharomyces cerevisiae
-
sake yeast strain Kyokai No. 7
-
Purification (Commentary)
Commentary
Organism
PBE 94 column, DEAE-cellulose, Toypearl HW60, hydroxyapatite, octyl Sepharose
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.47
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
isoamylalcohol + acetyl-CoA
slightly higher activity with straight-chain alcohols than with branched-chain alcohols, activity with straight-chain alcohols in descending order: pentanol, butanol, propanol, very low activity with ethanol, activity with branched-chain alcohols in descending order of carbon number: C5, C4, C3
487559
Saccharomyces cerevisiae
isoamyl acetate + CoA
-
487559
Saccharomyces cerevisiae
-
Subunits
Subunits
Commentary
Organism
?
x * 22000, SDS-PAGE
Cladosporium cladosporioides
?
x * 60000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Saccharomyces cerevisiae
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
10
-
loss of 20% activity
Saccharomyces cerevisiae
20
-
rapid inactivation above
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.9
-
-
Cladosporium cladosporioides
8
-
-
Saccharomyces cerevisiae
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
8
no loss of activity after 22 h at 4C
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
complete inhibition
Saccharomyces cerevisiae
Cd2+
-
Saccharomyces cerevisiae
Cu2+
-
Saccharomyces cerevisiae
Hg2+
-
Saccharomyces cerevisiae
linoleic acid
2 mM, 86% inhibition
Saccharomyces cerevisiae
linolenic acid
2 mM, 68% inhibition
Saccharomyces cerevisiae
p-chloromercuribenzoate
complete inhibition
Saccharomyces cerevisiae
Zn2+
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
acetyl-CoA
strain NCYC 366
Saccharomyces cerevisiae
0.19
-
acetyl-CoA
-
Saccharomyces cerevisiae
29.8
-
isoamyl alcohol
-
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
x * 22000, SDS-PAGE
Cladosporium cladosporioides
60000
-
x * 60000, SDS-PAGE
Saccharomyces cerevisiae
270000
-
gel filtration
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
PBE 94 column, DEAE-cellulose, Toypearl HW60, hydroxyapatite, octyl Sepharose
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.47
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
isoamylalcohol + acetyl-CoA
slightly higher activity with straight-chain alcohols than with branched-chain alcohols, activity with straight-chain alcohols in descending order: pentanol, butanol, propanol, very low activity with ethanol, activity with branched-chain alcohols in descending order of carbon number: C5, C4, C3
487559
Saccharomyces cerevisiae
isoamyl acetate + CoA
-
487559
Saccharomyces cerevisiae
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 22000, SDS-PAGE
Cladosporium cladosporioides
?
x * 60000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Saccharomyces cerevisiae
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
10
-
loss of 20% activity
Saccharomyces cerevisiae
20
-
rapid inactivation above
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.9
-
-
Cladosporium cladosporioides
8
-
-
Saccharomyces cerevisiae
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
8
no loss of activity after 22 h at 4C
Saccharomyces cerevisiae
Other publictions for EC 2.3.1.84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736721
Ding
The yeast ATF1 acetyltransfera ...
Saccharomyces cerevisiae
Lipids
51
469-475
2016
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6
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735555
Guo
Metabolic engineering of Esche ...
Saccharomyces cerevisiae
Appl. Microbiol. Biotechnol.
99
7805-7812
2015
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1
1
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3
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736188
Zhang
Acetate ester production by Ch ...
Saccharomyces cerevisiae
Genet. Mol. Res.
13
9735-9746
2014
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1
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1
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736589
Dong
Enhanced acetate ester product ...
Saccharomyces cerevisiae
J. Ind. Microbiol. Biotechnol.
41
1823-1828
2014
-
1
1
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4
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737129
Lin
Dual N- and C-terminal helices ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742
PLoS ONE
9
e104141
2014
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-
5
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3
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23
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5
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737039
Sharma
Biochemical characteristics of ...
Cymbopogon martinii
Plant Sci.
203-204
63-73
2013
-
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-
-
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-
3
6
-
1
1
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1
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1
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7
1
1
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6
1
1
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1
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3
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6
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1
1
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1
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7
1
1
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6
1
1
-
1
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6
6
720138
Cumplido-Laso
The fruit ripening-related gen ...
Fragaria x ananassa
J. Exp. Bot.
63
4275-4290
2012
-
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1
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17
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2
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1
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1
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1
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17
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1
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17
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2
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1
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1
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16
1
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17
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1
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1
25
25
719627
Balbontin
VpAAT1, a gene encoding an alc ...
Vasconcellea cundinamarcensis
J. Agric. Food Chem.
58
5114-5121
2010
-
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1
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1
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1
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1
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1
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14
1
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1
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1
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1
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1
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14
1
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1
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1
-
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684642
Van Laere
Flavour formation in fungi: ch ...
Kluyveromyces lactis
Appl. Microbiol. Biotechnol.
78
783-792
2008
-
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1
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8
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688115
Wang
Enzymatic characteristics of a ...
Hanseniaspora valbyensis
J. Food Biochem.
32
506-520
2008
1
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10
2
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1
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1
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1
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4
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1
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1
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1
1
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10
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2
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1
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1
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4
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1
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1
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1
1
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702712
Singh
Aerobic production of isoamyl ...
Saccharomyces cerevisiae
Bioprocess Biosyst. Eng.
31
299-306
2008
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1
1
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1
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1
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706467
Khanom
-
Bioconversion of aliphatic and ...
Cucumis melo
Postharvest Biol. Technol.
50
18-24
2008
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1
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1
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9
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9
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723419
Dexter
Characterization of a petunia ...
Petunia x hybrida
Plant J.
49
265-75
2007
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1
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8
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1
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1
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7
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8
1
1
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1
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8
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1
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7
-
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8
1
1
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1
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1
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675676
Wendakoon
Effect of short-term anaerobic ...
Musa acuminata
J. Sci. Food Agric.
86
1475-1480
2006
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676539
Guterman
Generation of phenylpropanoid ...
Rosa hybrid cultivar
Plant Mol. Biol.
60
555-563
2006
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1
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6
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4
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6
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677273
Lilly
The effect of increased yeast ...
Saccharomyces cerevisiae
Yeast
23
641-659
2006
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663447
Plata
-
Influence of glucose and oxyge ...
Saccharomyces cerevisiae
World J. Microbiol. Biotechnol.
21
115-121
2005
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1
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671295
Sharma
Trichloroacetic acid-facilitat ...
Cymbopogon martinii
Anal. Biochem.
346
176-178
2005
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1
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1
1
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1
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674960
Hirooka
Improved production of isoamyl ...
Saccharomyces cerevisiae
J. Biosci. Bioeng.
99
125-129
2005
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1
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676657
Defilippi
Apple aroma: alcohol acyltrans ...
Malus domestica
Plant Sci.
168
1199-1210
2005
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1
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1
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1
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Expression levels of the yeast ...
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Lilly
Effect of increased yeast alco ...
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Balance of activities of alcoh ...
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Alcohol acetyltransferase in t ...
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Partial purifiction and cataly ...
Wickerhamomyces anomalus
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Yoshioka
Ester formation by alcohol ace ...
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