Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-Gold cells | uncultured bacterium |
Crystallization (Comment) | Organism |
---|---|
in complex with sisomicin | uncultured bacterium |
meta-AAC0020 enzyme mutant Y138A in complex with sisomicin, sitting drop vapor diffusion method, using 20% (w/v) PEG3350, 0.2 M calcium acetate. The apoenzyme is crystallized in a solution containing 4 M sodium formate | uncultured bacterium |
Protein Variants | Comment | Organism |
---|---|---|
Y138A | catalytically inactive | uncultured bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
acetyl-CoA | K 0.5 value, Hill coefficient 1.77, pH 7.5, 25°C | uncultured bacterium | |
0.022 | - |
gentamycin | K 0.5 value, Hill coefficient 3.14, pH 7.5, 25°C | uncultured bacterium | |
0.028 | - |
tobramycin | K 0.5 value, Hill coefficient 2.3, pH 7.5, 25°C | uncultured bacterium | |
0.038 | - |
sisomicin | K 0.5 value, Hill coefficient 4.39, pH 7.5, 25°C | uncultured bacterium | |
0.346 | - |
kanamycin | K 0.5 value, Hill coefficient 0.62, pH 7.5, 25°C | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | A0A059WZ16 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex S200 gel filtration | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + gentamycin | - |
uncultured bacterium | CoA + ? | - |
? | |
acetyl-CoA + kanamycin | - |
uncultured bacterium | CoA + 6'-N-acetylkanamycin | - |
? | |
acetyl-CoA + kanamycin A | - |
uncultured bacterium | CoA + N6'-acetylkanamycin A | - |
? | |
acetyl-CoA + kanamycin C | - |
uncultured bacterium | CoA + N6'-acetylkanamycin C | - |
? | |
acetyl-CoA + sisomicin | - |
uncultured bacterium | CoA + N6'-acetylsisomicin | - |
? | |
acetyl-CoA + sisomicin | - |
uncultured bacterium | CoA + 6'-N-acetylsisomicin | - |
? | |
acetyl-CoA + tobramycin | - |
uncultured bacterium | CoA + N6'-acetyltobramycin | - |
? | |
acetyl-CoA + tobramycin | - |
uncultured bacterium | CoA + 6'-N-acetyltobramycin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAC | - |
uncultured bacterium |
AAC(6') | - |
uncultured bacterium |
AAC3-I | - |
uncultured bacterium |
aminoglycoside acetyltransferase | - |
uncultured bacterium |
aminoglycoside N-acetyltransferase | - |
uncultured bacterium |
meta-AAC0020 | - |
uncultured bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.207 | - |
kanamycin | pH 7.5, 25°C | uncultured bacterium | |
1.17 | - |
gentamycin | pH 7.5, 25°C | uncultured bacterium | |
1.2 | - |
tobramycin | pH 7.5, 25°C | uncultured bacterium | |
1.51 | - |
acetyl-CoA | pH 7.5, 25°C | uncultured bacterium | |
2.42 | - |
sisomicin | pH 7.5, 25°C | uncultured bacterium |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme confers high-level heterologous resistance to the aminoglycosides gentamicin C, sisomicin, and tobramycin | uncultured bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
kanamycin | K 0.5 value, Hill coefficient 0.62, pH 7.5, 25°C | uncultured bacterium | |
43 | - |
tobramycin | K 0.5 value, Hill coefficient 2.3, pH 7.5, 25°C | uncultured bacterium | |
52 | - |
gentamycin | K 0.5 value, Hill coefficient 3.14, pH 7.5, 25°C | uncultured bacterium | |
64 | - |
sisomicin | K 0.5 value, Hill coefficient 4.39, pH 7.5, 25°C | uncultured bacterium | |
69 | - |
acetyl-CoA | K 0.5 value, Hill coefficient 1.77, pH 7.5, 25°C | uncultured bacterium |