Protein Variants | Comment | Organism |
---|---|---|
E72A | mutation causes a notable reduction in affinity for both the 4,5- and 4,5-disubstituted classes of aminoglycosides, with the largest changes being an 83fold decrease in the affinity for the minimal substrate neamine and a 175fold reduction in Km-value for kanamycin | Enterococcus faecium |
H74A | kinetic parameters reveal only a small effect of the mutation on enzyme activity, Km-values for acetyl-CoA and the 4,5- and 4,6-disubstituted aminoglycosides only differs by a range of 2-7.5fold compared to that of the wild-type enzyme, indicating no significant change in apparent affinity | Enterococcus faecium |
L76A | mutant enzyme shows virtually wild-type activity, with the biggest changes being a 3.6fold reduction in the apparent affinity for neomycin and a 3.7fold decrease in turnover number for amikacin | Enterococcus faecium |
L76P | mutant enzyme is impaired in both aminoglycoside recognition and catalysis, 2300fold decrease in the ratio of turnover number to Kb-value for neamine, 1300fold reduction in the ratio of turnover number to Kb-value for tobramycin, no detectable activity towardskanamycin A and amikacin | Enterococcus faecium |
Y147A | no detectable activity towardsamikacin | Enterococcus faecium |
Y147F | decrease in aminoglycoside affinity ranges from a 6fold decrease for tobramycin to a large 170fold change in KM-value for amikacin | Enterococcus faecium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
acetyl-CoA | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
0.0041 | - |
acetyl-CoA | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
0.0047 | - |
acetyl-CoA | pH 6.0, 37°C, mutant enzyme Y147A | Enterococcus faecium | |
0.0053 | - |
neomycin | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
0.0058 | - |
neamine | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
0.0073 | - |
acetyl-CoA | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.0091 | - |
ribostamycin | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
0.0114 | - |
amikacin | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
0.0131 | - |
amikacin | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
0.0144 | - |
kanamycin A | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.0182 | - |
neomycin | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.019 | - |
neomycin | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
0.0199 | - |
kanamycin A | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
0.0214 | - |
ribostamycin | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
0.022 | - |
tobramycin | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
0.0224 | - |
neamine | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
0.0235 | - |
acetyl-CoA | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
0.0257 | - |
kanamycin A | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
0.029 | - |
neomycin | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium | |
0.0369 | - |
tobramycin | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.0381 | - |
ribostamycin | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.0386 | - |
neomycin | pH 6.0, 37°C, mutant enzyme Y147A | Enterococcus faecium | |
0.0432 | - |
neamine | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.0489 | - |
acetyl-CoA | pH 6.0, 37°C, mutant enzyme L76P | Enterococcus faecium | |
0.0553 | - |
neomycin | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
0.0573 | - |
neomycin | pH 6.0, 37°C, mutant enzyme L76P | Enterococcus faecium | |
0.0582 | - |
amikacin | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.0682 | - |
neamine | pH 6.0, 37°C, mutant enzyme L76P | Enterococcus faecium | |
0.107 | - |
ribostamycin | pH 6.0, 37°C, mutant enzyme L76P | Enterococcus faecium | |
0.109 | - |
ribostamycin | pH 6.0, 37°C, mutant enzyme Y147A | Enterococcus faecium | |
0.11 | - |
kanamycin A | pH 6.0, 37°C, mutant enzyme Y147A | Enterococcus faecium | |
0.136 | - |
tobramycin | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
0.146 | - |
tobramycin | pH 6.0, 37°C, mutant enzyme L76P | Enterococcus faecium | |
0.147 | - |
tobramycin | pH 6.0, 37°C, mutant enzyme Y147A | Enterococcus faecium | |
0.15 | - |
neamine | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
0.154 | - |
acetyl-CoA | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium | |
0.169 | - |
neamine | pH 6.0, 37°C, mutant enzyme Y147A | Enterococcus faecium | |
0.301 | - |
ribostamycin | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium | |
0.306 | - |
ribostamycin | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
0.34 | - |
kanamycin A | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
0.38 | - |
amikacin | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium | |
0.482 | - |
neamine | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium | |
0.62 | - |
tobramycin | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
1.15 | - |
tobramycin | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium | |
2.19 | - |
amikacin | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
3.49 | - |
kanamycin A | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecium | - |
chromosomally encoded aminoglycoside 6'-N-acetyltransferase | - |
Purification (Comment) | Organism |
---|---|
- |
Enterococcus faecium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + amikacin | - |
Enterococcus faecium | CoA + N6'-acetylamikacin | - |
? | |
acetyl-CoA + kanamycin A | - |
Enterococcus faecium | CoA + N6'-acetylkanamycin A | - |
? | |
acetyl-CoA + neamine | - |
Enterococcus faecium | CoA + N6'-acetylneamine | - |
? | |
acetyl-CoA + neomycin | - |
Enterococcus faecium | CoA + N6'-acetylneomycin | - |
? | |
acetyl-CoA + ribostamycin | - |
Enterococcus faecium | CoA + N6'-acetylribostamycin | - |
? | |
acetyl-CoA + tobramycin | - |
Enterococcus faecium | CoA + N6'-acetyltobramycin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminoglycoside 6'-N-acetyltransferase | - |
Enterococcus faecium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
neomycin | pH 6.0, 37°C, mutant enzyme Y147A | Enterococcus faecium | |
0.002 | - |
neamine | pH 6.0, 37°C, mutant enzyme L76P | Enterococcus faecium | |
0.003 | - |
amikacin | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
0.004 | - |
tobramycin | pH 6.0, 37°C, mutant enzyme Y147A | Enterococcus faecium | |
0.004 | - |
neamine | pH 6.0, 37°C, mutant enzyme Y147A | Enterococcus faecium | |
0.01 | - |
tobramycin | pH 6.0, 37°C, mutant enzyme L76P | Enterococcus faecium | |
0.01 | - |
ribostamycin | pH 6.0, 37°C, mutant enzyme L76P | Enterococcus faecium | |
0.01 | - |
acetyl-CoA | pH 6.0, 37°C, mutant enzyme Y147A | Enterococcus faecium | |
0.01 | - |
kanamycin A | pH 6.0, 37°C, mutant enzyme Y147A | Enterococcus faecium | |
0.02 | - |
ribostamycin | pH 6.0, 37°C, mutant enzyme Y147A | Enterococcus faecium | |
0.03 | - |
amikacin | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
0.03 | - |
kanamycin A | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
0.04 | - |
amikacin | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.04 | - |
neomycin | pH 6.0, 37°C, mutant enzyme L76P | Enterococcus faecium | |
0.05 | - |
amikacin | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium | |
0.05 | - |
kanamycin A | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.05 | - |
neamine | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
0.06 | - |
acetyl-CoA | pH 6.0, 37°C, mutant enzyme L76P | Enterococcus faecium | |
0.07 | - |
acetyl-CoA | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
0.07 | - |
neomycin | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
0.1 | - |
tobramycin | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
0.11 | - |
amikacin | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
0.12 | - |
ribostamycin | pH 6.0, 37°C, mutant enzyme Y147F | Enterococcus faecium | |
0.15 | - |
neamine | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.15 | - |
ribostamycin | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
0.16 | - |
neomycin | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
0.18 | - |
acetyl-CoA | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.19 | - |
neomycin | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.2 | - |
neomycin | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
0.23 | - |
ribostamycin | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.23 | - |
acetyl-CoA | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
0.27 | - |
kanamycin A | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
0.3 | - |
tobramycin | pH 6.0, 37°C, mutant enzyme H74A | Enterococcus faecium | |
0.34 | - |
ribostamycin | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
0.35 | - |
ribostamycin | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium | |
0.38 | - |
neamine | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
0.4 | - |
acetyl-CoA | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
0.42 | - |
neamine | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
0.44 | - |
neamine | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium | |
0.55 | - |
kanamycin A | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium | |
0.82 | - |
kanamycin A | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
0.89 | - |
tobramycin | pH 6.0, 37°C, mutant enzyme L76A | Enterococcus faecium | |
0.99 | - |
acetyl-CoA | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium | |
1.1 | - |
tobramycin | pH 6.0, 37°C, wild-type enzyme | Enterococcus faecium | |
1.1 | - |
tobramycin | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium | |
1.1 | - |
neomycin | pH 6.0, 37°C, mutant enzyme E72A | Enterococcus faecium |