General Stability | Organism |
---|---|
GTP, CoA, gentamicin C, neamine protect against thermal inactivation | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6'-N-acetylneamine | product inhibition | Staphylococcus aureus | |
AMP | - |
Staphylococcus aureus | |
CoA | product inhibition | Staphylococcus aureus | |
gentamicin C1 | competitive inhibitor of neamine, non-competitive inhibitor of acetyl-CoA | Staphylococcus aureus | |
additional information | no inhibition by GTP | Staphylococcus aureus | |
paromomycin | inhibits reaction with neamine | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
sucrose density gradient centrifugation | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Staphylococcus aureus | involved in resistance to antibiotics | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
harbouring plasmid RPAL | - |
Purification (Comment) | Organism |
---|---|
unseparable from 2-O-aminoglycoside phosphotransferase by affinity chromatography or sucrose density gradient ultracentrifugation | Staphylococcus aureus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + kanamycin-B = CoA + N6'-acetylkanamycin-B | mechanism | Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.304 | - |
- |
Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + gentamicin C1a | - |
Staphylococcus aureus | CoA + N6'-acetylgentamicin C1a | - |
? | |
acetyl-CoA + kanamycin B | - |
Staphylococcus aureus | CoA + N6'-acetylkanamycin B | - |
? | |
acetyl-CoA + neamine | - |
Staphylococcus aureus | CoA + 6'-N-acetylneamine | - |
? | |
acetyl-CoA + neamine | - |
Staphylococcus aureus | CoA + N6'-acetylneamine | - |
? | |
acetyl-CoA + tobramycin | - |
Staphylococcus aureus | CoA + N6'-acetyltobramycin | - |
? | |
additional information | involved in resistance to antibiotics | Staphylococcus aureus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
GTP, CoA, gentamicin C, neamine protect against thermal inactivation | Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
CoA | pH 6.9, 37°C | Staphylococcus aureus | |
4 | - |
gentamicin C1 | pH 6.9, 37°C, inhibition of the reaction with neamine | Staphylococcus aureus | |
6 | - |
AMP | pH 6.9, 37°C, inhibition of the reaction with neamine | Staphylococcus aureus | |
7.7 | - |
paromomycin | pH 6.9, 37°C, inhibition of the reaction with neamine | Staphylococcus aureus |