Cloned (Comment) | Organism |
---|---|
expressed in the pET-23a(+) expression vector | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
desulfo-CoA | - |
Escherichia coli | |
netilmicin | - |
Escherichia coli | |
ribostamycin | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
paromomycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
0.00033 | - |
sisomycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
0.00036 | - |
neomycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
0.00067 | - |
apramycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
0.00097 | - |
gentamycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
0.00142 | - |
tobramycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
0.0031 | - |
ribostamycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
0.042 | - |
netilmicin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
0.086 | - |
acetyl-CoA | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
0.237 | - |
butirosin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
0.238 | - |
kanamycin A | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
0.477 | - |
malonyl-CoA | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
0.499 | - |
propionyl-CoA | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
1.16 | - |
butyryl-CoA | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
11.07 | - |
amikacin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, enzyme in solution | Escherichia coli |
54000 | - |
dynamic light scattering | Escherichia coli |
55750 | - |
calculated from amino acid sequence | Escherichia coli |
60000 | - |
Sephacryl-S200 gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q306W4 | - |
- |
Escherichia coli BL21(DE3) pLysS | Q306W4 | - |
- |
Purification (Comment) | Organism |
---|---|
Fast Flow Q-Sepharose anion exchange column chromatography and Phenyl Sepharose column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + amikacin | - |
Escherichia coli | CoA + N3'-acetylamikacin | - |
? | |
acetyl-CoA + amikacin | - |
Escherichia coli BL21(DE3) pLysS | CoA + N3'-acetylamikacin | - |
? | |
acetyl-CoA + apramycin | - |
Escherichia coli | CoA + N3'-acetylapramycin | - |
? | |
acetyl-CoA + apramycin | - |
Escherichia coli BL21(DE3) pLysS | CoA + N3'-acetylapramycin | - |
? | |
acetyl-CoA + butirosin | - |
Escherichia coli | CoA + N3'-acetylbutirosin | - |
? | |
acetyl-CoA + gentamicin | - |
Escherichia coli | CoA + N3'-acetylgentamicin | - |
? | |
acetyl-CoA + gentamicin | - |
Escherichia coli BL21(DE3) pLysS | CoA + N3'-acetylgentamicin | - |
? | |
acetyl-CoA + kanamycin | - |
Escherichia coli | CoA + N3'-acetylkanamycin | - |
? | |
acetyl-CoA + kanamycin | - |
Escherichia coli BL21(DE3) pLysS | CoA + N3'-acetylkanamycin | - |
? | |
acetyl-CoA + neomycin | - |
Escherichia coli | CoA + ? | - |
? | |
acetyl-CoA + netilmicin | - |
Escherichia coli | CoA + N3'-acetylnetilmicin | - |
? | |
acetyl-CoA + paromomycin | - |
Escherichia coli | CoA + N3'-acetylparomomycin | - |
? | |
acetyl-CoA + ribostamycin | - |
Escherichia coli | CoA + ? | - |
? | |
acetyl-CoA + sisomycin | - |
Escherichia coli | CoA + N3'-acetylsisomycin | - |
? | |
acetyl-CoA + tobramycin | - |
Escherichia coli | CoA + N3'-acetyltobramycin | - |
? | |
acetyl-CoA + tobramycin | - |
Escherichia coli BL21(DE3) pLysS | CoA + N3'-acetyltobramycin | - |
? | |
butyryl-CoA + ribostamycin | - |
Escherichia coli | CoA + ? | - |
? | |
desulfo-CoA + ribostamycin | - |
Escherichia coli | ? | - |
? | |
malonyl-CoA + ribostamycin | - |
Escherichia coli | CoA + ? | - |
? | |
propionyl-CoA + ribostamycin | - |
Escherichia coli | CoA + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, enzyme in solution | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
AAC(3)-IV | - |
Escherichia coli |
AAC3-IV aminoglycoside acetyltransferase | - |
Escherichia coli |
aminoglycoside 3-N-acetyltransferase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
butyryl-CoA | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
0.5 | - |
amikacin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
3.5 | - |
butirosin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
9 | - |
malonyl-CoA | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
15 | - |
paromomycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
32 | - |
netilmicin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
33 | - |
propionyl-CoA | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
39 | - |
acetyl-CoA | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
40 | - |
gentamycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
51 | - |
sisomycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
60 | - |
neomycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
61 | - |
apramycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
80 | - |
kanamycin A | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
146 | - |
ribostamycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli | |
162 | - |
tobramycin | in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
corresponds to the plateau region of the pH profile, where the velocity is maximal and the variations in velocity due to changes in pH are smallest | Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | calculated from amino acid sequence | - |
5.7 |