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Literature summary for 2.3.1.8 extracted from

  • Yoshida, Y.; Sato, M.; Nonaka, T.; Hasegawa, Y.; Kezuka, Y.
    Characterization of the phosphotransacetylase-acetate kinase pathway for ATP production in Porphyromonas gingivalis (2019), J. Oral Microbiol., 11, 1588086 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Porphyromonas gingivalis

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.04 A resolution. An acetyl-CoA molecule binds to each subunit of the dimer Porphyromonas gingivalis

Protein Variants

Protein Variants Comment Organism
D309A complete loss of activity, mutation does not affect the dimerization Porphyromonas gingivalis
D318A complete loss of activity, mutant displays a broad biphasic pattern in gel filtration Porphyromonas gingivalis
R135A 21% decrease in specific activity, mutation does not affect the dimerization Porphyromonas gingivalis
R312A complete loss of activity, mutation does not affect the dimerization Porphyromonas gingivalis
R89A 58% decrease in specific activity, mutation does not affect the dimerization Porphyromonas gingivalis
S311A complete loss of activity, mutation does not affect the dimerization Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0638
-
 acetyl-CoA pH 9.0, 37°C Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80500
-
 gel filtration Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis B2RK03
-
-
Porphyromonas gingivalis ATCC 33277 B2RK03
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + carnitine
-
 Porphyromonas gingivalis CoA + O-acetylcarnitine
-
 ?
acetyl-CoA + carnitine
-
 Porphyromonas gingivalis ATCC 33277 CoA + O-acetylcarnitine
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 35800, SDS-PAGE and calculated from sequence Porphyromonas gingivalis

Synonyms

Synonyms Comment Organism
PGN_1179
-
 Porphyromonas gingivalis
PTA
-
 Porphyromonas gingivalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1487
-
 acetyl-CoA pH 9.0, 37°C Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Porphyromonas gingivalis

General Information

General Information Comment Organism
physiological function the phosphotransacetylase Pta and acetate kinase Ack genes are tandemly located in the genome, and are cotranscribed as an operon. Inactivation of Pta or Ack by homologous recombination is successful only when the inactivated gene is expressed in trans Porphyromonas gingivalis