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Literature summary for 2.3.1.8 extracted from

  • Taylor, T.; Ingram-Smith, C.; Smith, K.S.
    Biochemical and Kinetic Characterization of the eukaryotic phosphotransacetylase class IIa enzyme from Phytophthora ramorum (2015), Eukaryot. Cell, 14, 652-660.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.8

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol maxmimum activity in the presence of 1 mM dithiothreitol Phytophthora ramorum
pyruvate isoform PtaII has 133% activity in the presence of 0.05 mM pyruvate Phytophthora ramorum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2(DE3) pLysS cells Phytophthora ramorum

Protein Variants

Protein Variants Comment Organism
G300A the mutant of isoform PtaII shows reduced activity compared to the wild type enzyme Phytophthora ramorum
G300D the mutant of isoform PtaII shows reduced activity compared to the wild type enzyme Phytophthora ramorum

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Phytophthora ramorum
AMP complete loss of activity at 3 mM AMP Phytophthora ramorum
ATP
-
 Phytophthora ramorum
additional information fructose-1,6-bisphosphate (1 mM), oxaloacetate (1 mM), 2-oxoglutarate (1.5 mM), and citrate (1.5 mM) have no effect on enzyme activity Phytophthora ramorum
NAD+ potent inhibitor Phytophthora ramorum
NADH potent inhibitor Phytophthora ramorum
NADP+ potent inhibitor Phytophthora ramorum
NADPH potent inhibitor Phytophthora ramorum
phosphoenolpyruvate
-
 Phytophthora ramorum
pyruvate inhibitory at 0.5 mM Phytophthora ramorum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ maximum activity in the presence of 20 mM KCl Phytophthora ramorum
NH4+ maximum activity in the presence of 20 mM NH4Cl Phytophthora ramorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + phosphate Phytophthora ramorum
-
 CoA + acetyl phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Phytophthora ramorum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap HP Ni2+ affinity column chromatography Phytophthora ramorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + phosphate
-
 Phytophthora ramorum CoA + acetyl phosphate
-
 r

Synonyms

Synonyms Comment Organism
phosphotransacetylase
-
 Phytophthora ramorum
PTA
-
 Phytophthora ramorum
PtaII1 isoform Phytophthora ramorum
PtaII2 isoform Phytophthora ramorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 isoform PtaII1 Phytophthora ramorum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.052
-
 mutant enzyme G300D, at pH 8.0 and 37°C Phytophthora ramorum NADH
0.053
-
 mutant enzyme G300D, at pH 8.0 and 37°C Phytophthora ramorum AMP
0.074
-
 mutant enzyme G300D, at pH 8.0 and 37°C Phytophthora ramorum phosphoenolpyruvate
0.081
-
 mutant enzyme G300D, at pH 8.0 and 37°C Phytophthora ramorum NADP+
0.133
-
 mutant enzyme G300D, at pH 8.0 and 37°C Phytophthora ramorum ATP
0.135
-
 wild type enzyme, at pH 8.0 and 37°C Phytophthora ramorum NADP+
0.275
-
 wild type enzyme, at pH 8.0 and 37°C Phytophthora ramorum NADH
0.287
-
 wild type enzyme, at pH 8.0 and 37°C Phytophthora ramorum AMP
0.736
-
 wild type enzyme, at pH 8.0 and 37°C Phytophthora ramorum ATP
1.018
-
 wild type enzyme, at pH 8.0 and 37°C Phytophthora ramorum phosphoenolpyruvate