Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)/pSJS1244 | Streptococcus pyogenes |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Streptococcus pyogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + phosphate | Streptococcus pyogenes | - |
CoA + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + phosphate | - |
Streptococcus pyogenes | CoA + acetyl phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | crystal structure analysis | Streptococcus pyogenes |