Literature summary for 2.3.1.8 extracted from

Iyer, P.P.; Ferry, J.G.
Role of arginines in coenzyme A binding and catalysis by the phosphotransacetylase from Methanosarcina thermophila (2001), J. Bacteriol., 183, 4244-4250.

Search for more literature for 2.3.1.8

Protein Variants

Protein Variants	Comment	Organism
R133A	altered kinetic properties, increased Km for CoA	Methanosarcina thermophila
R133E	altered kinetic properties, increased Km for CoA	Methanosarcina thermophila
R133K	altered kinetic properties, increased Km for CoA	Methanosarcina thermophila
R133Q	altered kinetic properties, increased Km for CoA	Methanosarcina thermophila
R87A	altered kinetic properties, increased Km for CoA	Methanosarcina thermophila
R87E	altered kinetic properties, increased Km for CoA	Methanosarcina thermophila
R87K	altered kinetic properties, increased Km for CoA	Methanosarcina thermophila
R87Q	altered kinetic properties, increased Km for CoA	Methanosarcina thermophila

Inhibitors

Inhibitors	Comment	Organism	Structure
2,3-Butanedione	almost complete loss of wild-type enzyme activity after 10 min at 10 mM	Methanosarcina thermophila
desulfo-CoA	strong competitive inhibitor	Methanosarcina thermophila
phosphate	-	Methanosarcina thermophila

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.07	-	CoA	wild-type	Methanosarcina thermophila
0.11	-	CoA	R133K mutant	Methanosarcina thermophila
0.27	-	CoA	R87E mutant	Methanosarcina thermophila
0.36	-	CoA	R133A mutant	Methanosarcina thermophila
0.7	-	CoA	R133Q mutant	Methanosarcina thermophila
0.7	-	CoA	R133E mutant	Methanosarcina thermophila
0.75	-	CoA	R87K mutant	Methanosarcina thermophila
0.8	-	desulfo-CoA	R87E mutant	Methanosarcina thermophila
0.8	-	desulfo-CoA	R133K mutant	Methanosarcina thermophila
0.9	-	CoA	R87A mutant	Methanosarcina thermophila
1	-	desulfo-CoA	R133E mutant	Methanosarcina thermophila
1.1	-	CoA	R87Q mutant	Methanosarcina thermophila
1.3	-	desulfo-CoA	R87Q mutant	Methanosarcina thermophila
1.3	-	desulfo-CoA	R133A mutant	Methanosarcina thermophila
1.4	-	desulfo-CoA	wild-type	Methanosarcina thermophila
3.9	-	desulfo-CoA	R87K mutant	Methanosarcina thermophila
4	-	desulfo-CoA	R133Q mutant	Methanosarcina thermophila
6	-	desulfo-CoA	R87A mutant	Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + phosphate	Methanosarcina thermophila	-	CoA + acetyl phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Methanosarcina thermophila	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + phosphate	-	Methanosarcina thermophila	CoA + acetyl phosphate	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00467	-	desulfo-CoA	R133E mutant	Methanosarcina thermophila
0.0125	-	desulfo-CoA	R133A mutant	Methanosarcina thermophila
0.133	-	desulfo-CoA	R133K mutant	Methanosarcina thermophila
0.283	-	CoA	R87E mutant	Methanosarcina thermophila
0.383	-	desulfo-CoA	R87E mutant	Methanosarcina thermophila
0.467	-	desulfo-CoA	R133Q mutant	Methanosarcina thermophila
0.683	-	desulfo-CoA	R87K mutant	Methanosarcina thermophila
0.733	-	CoA	R133E mutant	Methanosarcina thermophila
1.63	-	desulfo-CoA	R87Q mutant	Methanosarcina thermophila
3.07	-	desulfo-CoA	R87A mutant	Methanosarcina thermophila
4.53	-	desulfo-CoA	wild-type	Methanosarcina thermophila
6.22	-	CoA	R87K mutant	Methanosarcina thermophila
6.97	-	CoA	R133K mutant	Methanosarcina thermophila
10.5	-	CoA	R133A mutant	Methanosarcina thermophila
19.1	-	CoA	R133Q mutant	Methanosarcina thermophila
20.4	-	CoA	R87A mutant	Methanosarcina thermophila
23.2	-	CoA	R87Q mutant	Methanosarcina thermophila
81.3	-	CoA	wild-type	Methanosarcina thermophila

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.001	-	desulfo-CoA	-	Methanosarcina thermophila
0.32	-	phosphate	R133Q mutant	Methanosarcina thermophila
0.52	-	phosphate	R87Q mutant	Methanosarcina thermophila
0.81	-	phosphate	wild-type enzyme	Methanosarcina thermophila

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Release 2023.1 (January 2023)