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Literature summary for 2.3.1.75 extracted from

  • Tomiyama, T.; Kurihara, K.; Ogawa, T.; Maruta, T.; Ogawa, T.; Ohta, D.; Sawa, Y.; Ishikawa, T.
    Wax ester synthase/diacylglycerol acyltransferase isoenzymes play a pivotal role in wax ester biosynthesis in Euglena gracilis (2017), Sci. Rep., 7, 13504 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.75

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain H1246 Euglena gracilis
gene WSD2, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons and phylogenetic analysis, analysis of the molecular organization of six WSD isoforms in Euglena gracilis, quantitative PCR enzyme expression analysis in wild-type and mutant cells Euglena gracilis
gene WSD5, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons and phylogenetic analysis, analysis of the molecular organization of six WSD isoforms in Euglena gracilis, quantitative PCR enzyme expression analysis in wild-type and mutant cells Euglena gracilis

Protein Variants

Protein Variants Comment Organism
additional information generation of single EgWSD2 and double knockout EgWSD2/5 mutant cells, phenotypes, overview Euglena gracilis
additional information generation of single EgWSD5 and double knockout EgWSD2/5 mutant cells, phenotypes, overview Euglena gracilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + a long-chain alcohol Euglena gracilis
-
 CoA + a long-chain ester
-
 ?

Organism

Organism UniProt Comment Textmining
Euglena gracilis A0A1E1GJC5
-
-
Euglena gracilis A0A1E1GJD2
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + a long-chain alcohol
-
 Euglena gracilis CoA + a long-chain ester
-
 ?
additional information isoform WSD2 has a high specificity toward shorter chain length fatty acids and alcohols and readily utilize C14 fatty acid and C14 alcohol as substrates, show low activity with C16 substrates, and almost negligible reaction toward C18 substrates Euglena gracilis ?
-
-
additional information isoform WSD5 has a high specificity toward shorter chain length fatty acids and alcohols and readily utilize C14 fatty acid and C14 alcohol as substrates, show low activity with C16 substrates, and almost negligible reaction toward C18 substrates Euglena gracilis ?
-
-
additional information no activity with stearoyl-CoA and octadecanol as substrates Euglena gracilis ?
-
-
myristoyl-CoA + tetradecanol 100% conversion efficiency Euglena gracilis myristyl myristate + CoA
-
 ?
palmitoyl-CoA + hexadecanol about 25% conversion efficiency compared to myristoyl-CoA plus tetradecanol Euglena gracilis palmityl palmitate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
EgWSD2
-
 Euglena gracilis
EgWSD5
-
 Euglena gracilis
More cf. EC 2.3.1.20 Euglena gracilis
wax ester synthase/diacylglycerol acyltransferase
-
 Euglena gracilis
wax ester synthase/diacylglycerol acyltransferase bifunctional enzyme Euglena gracilis
WS/DGAT
-
 Euglena gracilis
WSD2 isoform Euglena gracilis
WSD5 isoform Euglena gracilis

General Information

General Information Comment Organism
evolution among six possible predicted Euglena WSD orthologues, gene expression analysis and in vivo evaluation for enzyme activity with yeast expressing individual recombinant WSDs indicates that two of them, EgWSD2 and EgWSD5, predominantly function as wax ester synthase. The six WSD isoforms in Euglena gracilis all contain WES and DUF1298 motifs Euglena gracilis
malfunction remarkably reduced wax ester contents in EgWSD2/5-double knockdown Euglena gracilis cells treated with anaerobic conditions. The decreased ability to produce wax ester does not affect adaptation of Euglena gracilis to anaerobiosis. Lipid profile analysis suggests an allocation of metabolites to other compounds including triacylglycerol instead of wax esters, lipid profile analyses of EgWSD2/5 double-knockout cells, effect of EgWSD gene silencing on wax ester production, overview. The relative amount of wax esters, including C28, is significantly low in EgWSD2/5 double-KD cells, whereas that of fatty acids does not differ between the silenced cells and mock control cells Euglena gracilis
metabolism the enzyme plays a pivotal role in wax ester synthesis Euglena gracilis
additional information quantitative expression analysis of WS and WSD genes in response to aerobic and anaerobic conditions Euglena gracilis
physiological function pivotal role of both EgWSD2 and EgWSD5 in wax ester synthesis Euglena gracilis