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Literature summary for 2.3.1.75 extracted from
- Key enzymes for biosynthesis of neutral lipid storage compounds in prokaryotes: Properties, function and occurrence of wax ester synthases/acyl-CoA:diacylglycerol acyltransferases (2007), Biochimie, 89, 230-242.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene atfA, DNA and amino acid sequence determination and analysis, WS/DGAT family sequence comparisons and phylogenetic relationships, overview, expression in Escherichia coli as untagged or C-terminally His6-tagged enzyme | Acinetobacter baylyi |
| genes with high homologies to the Acinetobacter sp. ADP1 atfA are identified in the genome databases of several Gram-negative strains, including marine bacteria like Hahella chejuensis, psychrophilic strains like Psychrobacter sp., Polaromonas sp. and Bradyrhizobium japonicum. Ten atfA homologous genes are identified in the genome database of Arabidopsis thaliana | Acinetobacter baylyi ADP1 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of knockout mutants by transposon mutagenesis | Acinetobacter baylyi |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lipid body | - |
Acinetobacter baylyi | - |
- |
| membrane | - |
Acinetobacter baylyi ADP1 | 16020 | - |
| membrane | - |
Acinetobacter baylyi | 16020 | - |
| additional information | the enzyme exhibits a very specific adsorption pattern to artificial phospholipid membranes | Acinetobacter baylyi | - |
- |
| plasma membrane | the enzyme is located mainly on the membrane's cytosolic site and on the surface of intracellular wax ester inclusions, it possesses one putative predicted membrane-spanning region in hydrophobicity analysis | Acinetobacter baylyi | 5886 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51800 | - |
x * 51800, calculated from sequence | Acinetobacter baylyi ADP1 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Acinetobacter baylyi | key enzyme in the biosynthesis of neutral lipid storage compounds, triacylglycerols and wax esters, overview | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter baylyi | - |
i.e. Acinetobacter calcoaceticus, strain ADP1 | - |
| Acinetobacter baylyi ADP1 | Q8GGG1 | bifunctional protein | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli by cation exchange chromatography, hydrophobic interaction chromatography and subsequent anion exchange chromatography or by means of a C-terminal His6 tag | Acinetobacter baylyi |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acyl-CoA + a long-chain alcohol = CoA + a long-chain ester | reaction mechanism | Acinetobacter baylyi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.275 | - |
purified His-tagged recombinant enzyme, with palmitoyl-CoA | Acinetobacter baylyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + long-chain diacylglycerol | - |
Acinetobacter baylyi | CoA + long-chain triacylglycerol | - |
? | |
| acyl-CoA + long-chain fatty alcohol | - |
Acinetobacter baylyi | CoA + long-chain fatty ester | - |
? | |
| additional information | key enzyme in the biosynthesis of neutral lipid storage compounds, triacylglycerols and wax esters, overview | Acinetobacter baylyi | ? | - |
? | |
| additional information | a bifunctional enzyme also catalyzing the reaction of EC 2.3.1.20 | Acinetobacter baylyi | ? | - |
? | |
| palmitoyl-CoA + long-chain fatty alcohol | - |
Acinetobacter baylyi | CoA + long-chain palmitoyl ester | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 51800, calculated from sequence | Acinetobacter baylyi ADP1 |
| More | the enzyme contains the highly conserved HHXXXDG motif, amino acids 133-138, which may be involved in fatty acyl-CoA binding or catalytically participate in the acyltransferase reaction | Acinetobacter baylyi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtfA | - |
Acinetobacter baylyi ADP1 |
| AtfA | - |
Acinetobacter baylyi |
| More | cf. EC 2.3.1.20, the enzyme belongs to the WS/DGAT family | Acinetobacter baylyi |
| wax ester synthases/acyl-CoA:diacylglycerol acyltransferase | - |
Acinetobacter baylyi ADP1 |
| WE synthases/acyl-CoA:diacylglycerol acyltransferase | - |
Acinetobacter baylyi |
| WS/DGAT | - |
Acinetobacter baylyi ADP1 |
| WS/DGAT | - |
Acinetobacter baylyi |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Acinetobacter baylyi ADP1 | calculated from sequence | - |
9.05 |
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