Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | 45 mM, 10% increase in activtiy | Acinetobacter baylyi ADP1 |
General Stability | Organism |
---|---|
decrease in activity with buffers such as Tris, Bis-Tris, HEPES, morpholinoethanesulfonic acid | Acinetobacter baylyi ADP1 |
highly sensitive to proteases, especially to metallorpoteases, proteolysis is inhibited by addition of a cocktail of protease inhibitors including EDTA | Acinetobacter baylyi ADP1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 5 mM, 30% inhibition | Acinetobacter baylyi ADP1 | |
MgCl2 | 5 mM, 17% inhibition | Acinetobacter baylyi ADP1 | |
MnCl2 | 5 mM, 30% inhibition | Acinetobacter baylyi ADP1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
palmitoyl-CoA | pH 7.4, 35°C | Acinetobacter baylyi ADP1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | partially | Acinetobacter baylyi ADP1 | 5737 | - |
lipid droplet | - |
Acinetobacter baylyi ADP1 | 5811 | - |
membrane | cytoplasm membrane | Acinetobacter baylyi ADP1 | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE | Acinetobacter baylyi ADP1 |
94100 | - |
gel filtration | Acinetobacter baylyi ADP1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baylyi ADP1 | Q8GGG1 | bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, EC 2.3.1.75 and EC 2.3.1.20 resp., enzyme also has acyl-CoA:monoacylglycerol acyltransferase activity, expression in Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
pH 7.4, 35°C | Acinetobacter baylyi ADP1 |
Storage Stability | Organism |
---|---|
-70°C, several months without significant loss of activity | Acinetobacter baylyi ADP1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-decanol + palmitoyl-CoA | 48% the rate of hexacedanol | Acinetobacter baylyi ADP1 | decyl palmitate + CoA | - |
? | |
2-cyclohexylethanol + palmitoyl-CoA | 44.8% the rate of hexacedanol | Acinetobacter baylyi ADP1 | 2-cyclohexylethyl palmitate + CoA | - |
? | |
2-decanol + palmitoyl-CoA | 38.8% the rate of hexacedanol | Acinetobacter baylyi ADP1 | dec-2-yl palmitate + CoA | - |
? | |
4-decanol + palmitoyl-CoA | 15.6% the rate of hexacedanol | Acinetobacter baylyi ADP1 | 4-decyl palmitate + CoA | - |
? | |
cyclododecanol + palmitoyl-CoA | 79.7% the rate of hexacedanol | Acinetobacter baylyi ADP1 | cyclododecyl palmitate + CoA | - |
? | |
cyclohexandiol + palmitoyl-CoA | 4.1% the rate of hexacedanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
cyclohexanol + palmitoyl-CoA | 32% the rate of hexacedanol | Acinetobacter baylyi ADP1 | cyclohexyl palmitate + CoA | - |
? | |
cyclohexanone oxime + palmitoyl-CoA | 5.2% the rate of hexacedanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
hexadecanol + palmitoyl-CoA | - |
Acinetobacter baylyi ADP1 | hexadecyl palmitate + CoA | - |
? | |
additional information | no substrate: resorcinol | Acinetobacter baylyi ADP1 | ? | - |
? | |
phenol + palmitoyl-CoA | 4.1% the rate of hexacedanol | Acinetobacter baylyi ADP1 | phenyl palmitate + CoA | - |
? | |
phenylethanol + palmitoyl-CoA | 99% the rate of hexacedanol | Acinetobacter baylyi ADP1 | phenylethyl palmitate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE | Acinetobacter baylyi ADP1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Acinetobacter baylyi ADP1 |
65 | - |
no activity above | Acinetobacter baylyi ADP1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 40 | stable | Acinetobacter baylyi ADP1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
phosphate buffer | Acinetobacter baylyi ADP1 |