Literature summary for 2.3.1.74 extracted from

Kontturi, J.; Osama, R.; Deng, X.; Bashandy, H.; Albert, V.A.; Teeri, T.H.
Functional characterization and expression of GASCL1 and GASCL2, two anther-specific chalcone synthase like enzymes from Gerbera hybrida (2017), Phytochemistry, 134, 38-45 .

Search for more literature for 2.3.1.74

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli DH5alpha cells	Gerbera hybrid cultivar

Organism

Organism	UniProt	Comment	Textmining
Gerbera hybrid cultivar	A0A1D9C6D7	-	-

Purification (Commentary)

Purification (Comment)	Organism
His SpinTrap column chromatography	Gerbera hybrid cultivar

Source Tissue

Source Tissue	Comment	Organism	Textmining
anther	highest expression in tapetal cells of the anther	Gerbera hybrid cultivar	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 malonyl-CoA + 2 stearoyl-CoA	-	Gerbera hybrid cultivar	6-heptadecyl-4-hydroxy-2H-pyran-2-one + 4-hydroxy-6-(2-oxononadecyl)-2H-pyran-2-one + 2 CoA + 2 CO2	-	?
malonyl-CoA + acetyl-CoA	the reaction is catalyzed by isoform ASCL2 only	Gerbera hybrid cultivar	4-hydroxy-6-methyl-2-pyrone + CoA + CO2	-	?
malonyl-CoA + hexanoyl-CoA	-	Gerbera hybrid cultivar	4-hydroxy-6-pentyl-2H-pyran-2-one + CoA + CO2	-	?
malonyl-CoA + octanoyl-CoA	-	Gerbera hybrid cultivar	6-heptyl-4-hydroxy-2H-pyran-2-one + CoA + CO2	-	?
additional information	neither isoform GASCL1 nor isoform GASCL2 accept 4-coumaroyl-CoA or benzoyl-CoA as substrates	Gerbera hybrid cultivar	?	-	-

Subunits

Subunits	Comment	Organism
?	x * 43000, SDS-PAGE	Gerbera hybrid cultivar

Synonyms

Synonyms	Comment	Organism
anther-specific chalcone synthase-like enzyme	-	Gerbera hybrid cultivar
ASCL1	-	Gerbera hybrid cultivar
ASCL2	-	Gerbera hybrid cultivar

General Information

General Information	Comment	Organism
metabolism	the enzyme participates in sporopollenin biosynthesis and exine formation within the locule	Gerbera hybrid cultivar

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Release 2023.1 (January 2023)