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Literature summary for 2.3.1.74 extracted from

  • Shinozaki, J.; Kenmoku, H.; Nihei, K.; Masuda, K.; Noji, M.; Konno, K.; Asakawa, Y.; Kazuma, K.
    Cloning and functional analysis of three chalcone synthases from the flowers of safflowers Carthamus tinctorius (2016), Nat. Prod. Commun., 11, 787-790 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Carthamus tinctorius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 malonyl-CoA + 4-coumaroyl-CoA Carthamus tinctorius
-
4 CoA + naringenin chalcone + 3 CO2
-
?

Organism

Organism UniProt Comment Textmining
Carthamus tinctorius A0A1B4XSV2
-
-
Carthamus tinctorius A0A1B4XSV4
-
-
Carthamus tinctorius A0A1B4XSV6
-
-

Purification (Commentary)

Purification (Comment) Organism
MidiTrap G-25 column chromatography Carthamus tinctorius

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Carthamus tinctorius
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 malonyl-CoA + 4-coumaroyl-CoA
-
Carthamus tinctorius 4 CoA + naringenin chalcone + 3 CO2
-
?
3 malonyl-CoA + caffeoyl-CoA
-
Carthamus tinctorius ?
-
?
3 malonyl-CoA + feruloyl-CoA
-
Carthamus tinctorius ?
-
?
3 malonyl-CoA + sinapoyl-CoA
-
Carthamus tinctorius ?
-
?
malonyl-CoA + caffeoyl-CoA
-
Carthamus tinctorius ?
-
?
malonyl-CoA + feruloyl-CoA
-
Carthamus tinctorius ?
-
?
malonyl-CoA + sinapoyl-CoA
-
Carthamus tinctorius ?
-
?

Subunits

Subunits Comment Organism
? x * 42800, isoform CHS3, calculated from amino acid sequence Carthamus tinctorius
? x * 43400, isoform CHS1, calculated from amino acid sequence Carthamus tinctorius
? x * 43600, isoform CHS2, calculated from amino acid sequence Carthamus tinctorius

Synonyms

Synonyms Comment Organism
Chs1
-
Carthamus tinctorius
Chs2
-
Carthamus tinctorius
CHS3
-
Carthamus tinctorius