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Literature summary for 2.3.1.74 extracted from

  • Guo, H.; Yang, Y.; Ma, Y.; Liu, W.; Feng, J.; Luo, Z.; Lu, H.; Liu, C.; Yang, M.; Wang, Y.; Ma, L.
    A bifunctional type III polyketide synthase from raspberry (Rubus idaeus L.) with both chalcone synthase and benzalacetone synthase activity (2017), J. Plant Biochem. Biotechnol., 26, 80-90 .
No PubMed abstract available
Search for more literature for 2.3.1.74

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta-gami (DE3) and BL21-Rosetta (DE3) cells Rubus idaeus
overexpressed in Escherichia coli Rubus idaeus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01642
-
 4-coumaroyl-CoA pH 7.5, 35°C Rubus idaeus
0.01642
-
 4-coumaroyl-CoA at pH 7.5 and 40°C Rubus idaeus
0.02283
-
 malonyl-CoA at pH 7.5 and 40°C Rubus idaeus
0.2283
-
 malonyl-CoA pH 9.5, 35°C Rubus idaeus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42800
-
 calculated from sequence Rubus idaeus
43000
-
 SDS-PAGE Rubus idaeus
89000
-
 gel filtration Rubus idaeus

Organism

Organism UniProt Comment Textmining
Rubus idaeus Q8L5L2
-
-
Rubus idaeus Q9AU11
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rubus idaeus
Ni-NTA column chromatography Rubus idaeus

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Rubus idaeus
-
leaf young Rubus idaeus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 malonyl-CoA + 4-coumaroyl-CoA the enzyme effectively yields naringenin as a dominant product at pH 7.5 Rubus idaeus 4 CoA + naringenin chalcone + 3 CO2
-
 ?
3 malonyl-CoA + 4-coumaroyl-CoA when incubated with 4-coumaroyl-CoA and malonyl-CoA as substrates at pH 7.5, the enzyme catalyzes the formation of naringeninchalcone as a major product, along with 4-hydroxybenzalacetone. At pH 9.5, the enzyme effectively yields 4-hydroxybenzalacetone as a dominant product, along with naringenin chalcone Rubus idaeus 4 CoA + naringenin chalcone + 3 CO2
-
 ?
benzoyl-CoA + malonyl-CoA 22% activity compared to 4-coumaroyl-CoA Rubus idaeus ?
-
 ?
caffeoyl-CoA + malonyl-CoA 43% activity compared to 4-coumaroyl-CoA Rubus idaeus ?
-
 ?
cinnamoyl-CoA + malonyl-CoA 81% activity compared to 4-coumaroyl-CoA Rubus idaeus ?
-
 ?
feruloyl-CoA + malonyl-CoA 52% activity compared to 4-coumaroyl-CoA Rubus idaeus ?
-
 ?
additional information 4-coumaroyl-CoA and feruloyl-CoA are the only cinnamoyl-CoA derivatives accepted as starter substrates. RinPKS1 does not accept isobutyryl-CoA, isovaleryl-CoA or acetyl-CoA as substrates Rubus idaeus ?
-
-
additional information the enzyme does not accept isobutyryl-CoA, isovaleryl-CoA or acetyl-CoA as substrates Rubus idaeus ?
-
-

Subunits

Subunits Comment Organism
homodimer 2 * 43000, SDS-PAGE Rubus idaeus
homodimer 2 * 42800, calculated from amino acid sequence Rubus idaeus
homodimer 2 * S43000, SDS-PAGE Rubus idaeus

Synonyms

Synonyms Comment Organism
CHS6
-
 Rubus idaeus
PKS1 bifunctional enzyme with both chalcone synthase and benzalacetone synthase activity Rubus idaeus
RinPKS1 bifunctional enzyme with both chalcone synthase and benzalacetone synthase activity Rubus idaeus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Rubus idaeus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.045
-
 4-coumaroyl-CoA pH 9.5, 35°C Rubus idaeus
0.045
-
 4-coumaroyl-CoA at pH 7.5 and 40°C Rubus idaeus
0.071
-
 malonyl-CoA pH 9.5, 35°C Rubus idaeus
0.071
-
 malonyl-CoA at pH 7.5 and 40°C Rubus idaeus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rubus idaeus

pI Value

Organism Comment pI Value Maximum pI Value
Rubus idaeus calculated from amino acid sequence
-
 6
Rubus idaeus calculated from sequence
-
 6.04

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.742
-
 4-coumaroyl-CoA pH 9.5, 35°C Rubus idaeus
2.742
-
 4-coumaroyl-CoA at pH 7.5 and 40°C Rubus idaeus
3.122
-
 malonyl-CoA pH 9.5, 35°C Rubus idaeus
3.122
-
 malonyl-CoA at pH 7.5 and 40°C Rubus idaeus