Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method using buffered protein solutions (10-15 mg/ml) mixed with equal volumes of the reservoir solution and incubated at 4°C. Crystal structures of biphenyl synthase from Malus domestica and benzophenone synthase from Hypericum androsaemum are compared with the structure of an archetypal type III polyketide synthase - chalcone synthase from Malus domestica | Malus domestica |
hanging-drop vapor-diffusion method, incubation at 4°C. Crystal structures of biphenyl synthase from Malusx02domestica and benzophenone synthase from Hypericum androsaemum are compared with the structure of an archetypal type III polyketide synthase: chalcone synthase from Malus domestica. Both biphenyl synthase and benzophenone synthase contain mutations that reshape their active-site cavities to prevent the binding of 4-coumaroyl-CoA and to favor the binding of small hydrophobic substrates. The active-site cavities of biphenyl synthase and benzophenone synthase also contain a novel pocket associated with their chain-elongation and cyclization reactions | Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0128 | - |
malonyl-CoA | pH 7.0, 37°C | Malus domestica | |
0.0179 | - |
4-coumaroyl-CoA | pH 7.0, 37°C | Malus domestica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 malonyl-CoA + 4-coumaroyl-CoA | Malus domestica | - |
4 CoA + naringenin chalcone + 3 CO2 | - |
? | |
3 malonyl-CoA + benzoyl-CoA | Malus domestica | - |
4 CoA + 2,4,6-trihydroxybenzophenone + 3 CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | K9MUA0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 malonyl-CoA + 4-coumaroyl-CoA | - |
Malus domestica | 4 CoA + naringenin chalcone + 3 CO2 | - |
? | |
3 malonyl-CoA + benzoyl-CoA | - |
Malus domestica | 4 CoA + 2,4,6-trihydroxybenzophenone + 3 CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CHS2 chalcone synthase | - |
Malus domestica |
MdCHS2 | - |
Malus domestica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
4-coumaroyl-CoA | pH 7.0, 37°C | Malus domestica | |
0.0202 | - |
4-coumaroyl-CoA | pH 7.0, 37°C | Malus domestica |
General Information | Comment | Organism |
---|---|---|
evolution | crystal structures of biphenyl synthase from Malus domestica and benzophenone synthase from Hypericum androsaemum are compared with the structure of an archetypal type III polyketide synthase - chalcone synthase from Malus domestica. The results illuminate structural determinants of benzoic acid-specific type III PKSs and expand the understanding of the evolution of specialized metabolic pathways in plants | Malus domestica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
4-coumaroyl-CoA | pH 7.0, 37°C | Malus domestica | |
1.117 | - |
benzoyl-CoA | pH 7.0, 37°C | Malus domestica |