Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | indispensable for activity | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Digitonin | effective inhibitor | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | differential centrifugation of the leaf homogenate shows the 20000 * g pellet to have the highest amount of enzyme of the fractions tested but the highest specific activity is in the 88000 * g pellet. The active fractions contain endoplasmic reticulum, ribosomes and other membrane vesicles | Spinacia oleracea | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0002 | - |
leaf homogenate | Spinacia oleracea |
0.0014 | - |
20000 * g pellet from differential centrifugation of the leaf homogenate | Spinacia oleracea |
0.0022 | - |
88000 * g pellet from differential centrifugation of the leaf homogenate | Spinacia oleracea |
Storage Stability | Organism |
---|---|
-15°C, in a desiccator, several weeks | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-diacyl-sn-glycerol + sterol | - |
Spinacia oleracea | monoacylglycerol + sterol ester | - |
? | |
campesterol + 1,2-diacyl-sn-glycerol | - |
Spinacia oleracea | monoacylglycerol + campesterol-3-O-acyl ester | - |
? | |
cholesterol + 1,2-diacyl-sn-glycerol | - |
Spinacia oleracea | monoacylglycerol + cholesterol-3-O-acyl ester | - |
? | |
diacylglycerol + 1,2-diacyl-sn-glycerol | - |
Spinacia oleracea | monoacylglycerol + triacyl-sn-glycerol | - |
? | |
additional information | testing of diacylglycerols with only one type of fatty acid, preference for fatty acid transfer | Spinacia oleracea | ? | - |
? | |
additional information | monoacylglycerol and triacylglycerol are not suitable substrates | Spinacia oleracea | ? | - |
? | |
sitosterol + 1,2-diacyl-sn-glycerol | - |
Spinacia oleracea | monoacylglycerol + sitosterol-3-O-acyl ester | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
MES-NaOH buffer | Spinacia oleracea |