Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain InvSc1 and Escherichia coli strain BL21-CodonPlus(DE3)-RIL | Oryza sativa Japonica Group |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenylmethylsulfonyl fluoride | - |
Oryza sativa Japonica Group |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.83 | - |
myo-inositol | recombinant enzyme from Saccharomyces cerevisiae, at pH 7.4 and 30°C | Oryza sativa Japonica Group | |
1 | - |
myo-inositol | recombinant enzyme from Escherichia coli, at pH 7.4 and 30°C | Oryza sativa Japonica Group | |
1.38 | - |
myo-inositol | native enzyme, at pH 7.4 and 30°C | Oryza sativa Japonica Group |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-(indol-3-yl)acetyl-beta-D-glucose + myo-inositol | Oryza sativa Japonica Group | - |
D-glucose + O-(indol-3-yl)acetyl-myo-inositol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | B9EZV5 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | glycosylation is not necessary for enzyme activity | Oryza sativa Japonica Group |
Purification (Comment) | Organism |
---|---|
ConA-agarose column chromatography | Oryza sativa Japonica Group |
Renatured (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli, using 50 mM Tris-HCl buffer pH 8.5 containing 9.6 mM NaCl, 0.4 mM KCl, 2 mM MgCl2, 2 mM CaCl2, 0.5M arginin, 0.4 M sucrose, 0.75 mM guanidine hydrochloride, 1 mM GSH and 0.1 mM GSSH | Oryza sativa Japonica Group |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Oryza sativa Japonica Group | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00501 | - |
native enzyme, at pH 7.4 and 30°C | Oryza sativa Japonica Group |
0.01809 | - |
recombinant enzyme from Escherichia coli, at pH 7.4 and 30°C | Oryza sativa Japonica Group |
0.01875 | - |
recombinant enzyme from Saccharomyces cerevisiae, at pH 7.4 and 30°C | Oryza sativa Japonica Group |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-(indol-3-yl)acetyl-beta-D-glucose + myo-inositol | - |
Oryza sativa Japonica Group | D-glucose + O-(indol-3-yl)acetyl-myo-inositol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54300, native enzyme, calculated from amino acid sequence | Oryza sativa Japonica Group |
? | x * 54500, recombinant enzyme from Saccharomyces cerevisiae, calculated from amino acid sequence | Oryza sativa Japonica Group |
? | x * 58000, recombinant enzyme from Escherichia coli, calculated from amino acid sequence | Oryza sativa Japonica Group |
? | x * 58000, recombinant enzyme from Escherichia coli, SDS-PAGE | Oryza sativa Japonica Group |
? | x * 71000, native enzyme or recombinant enzyme from Saccharomyces cerevisiae, SDS-PAGE | Oryza sativa Japonica Group |
Synonyms | Comment | Organism |
---|---|---|
1-O-indole-3-acetyl-beta-D-glucose: myo-inositol acyltransferase | - |
Oryza sativa Japonica Group |
IAA-At | - |
Oryza sativa Japonica Group |
IAInos synthase | - |
Oryza sativa Japonica Group |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Oryza sativa Japonica Group |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Oryza sativa Japonica Group | native enzyme, calculated from amino acid sequence | - |
5.5 |
Oryza sativa Japonica Group | recombinant enzyme from Saccharomyces cerevisiae, calculated from amino acid sequence | - |
5.5 |
Oryza sativa Japonica Group | recombinant enzyme from Escherichia coli, calculated from amino acid sequence | - |
6 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
2.2 | - |
recombinant enzyme from Escherichia coli, in the presence of 4.4 mM myo-inositol, at pH 7.4 and 30°C | Oryza sativa Japonica Group | phenylmethylsulfonyl fluoride | |
2.5 | - |
recombinant enzyme from Saccharomyces cerevisiae, in the presence of 4.4 mM myo-inositol, at pH 7.4 and 30°C | Oryza sativa Japonica Group | phenylmethylsulfonyl fluoride | |
3.7 | - |
native enzyme, in the presence of 4.4 mM myo-inositol, at pH 7.4 and 30°C | Oryza sativa Japonica Group | phenylmethylsulfonyl fluoride | |
4 | - |
recombinant enzyme from Escherichia coli, in the presence of 8.1 mM myo-inositol, at pH 7.4 and 30°C | Oryza sativa Japonica Group | phenylmethylsulfonyl fluoride | |
4 | - |
recombinant enzyme from Saccharomyces cerevisiae, in the presence of 8.1 mM myo-inositol, at pH 7.4 and 30°C | Oryza sativa Japonica Group | phenylmethylsulfonyl fluoride | |
6.7 | - |
native enzyme, in the presence of 8.1 mM myo-inositol, at pH 7.4 and 30°C | Oryza sativa Japonica Group | phenylmethylsulfonyl fluoride |