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Literature summary for 2.3.1.7 extracted from

  • Altamimi, T.R.; Thomas, P.D.; Darwesh, A.M.; Fillmore, N.; Mahmoud, M.U.; Zhang, L.; Gupta, A.; Al Batran, R.; Seubert, J.M.; Lopaschuk, G.D.
    Cytosolic carnitine acetyltransferase as a source of cytosolic acetyl-CoA a possible mechanism for regulation of cardiac energy metabolism (2018), Biochem. J., 475, 959-976 .
    View publication on PubMed
Search for more literature for 2.3.1.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
 carnitine liver homogenate, pH 8.0, temperature not specified in the publication Mus musculus
0.011
-
 carnitine mitochondria from liver, pH 8.0, temperature not specified in the publication Mus musculus
0.0328
-
 CoA mitochondria from heart, pH 8.0, temperature not specified in the publication Mus musculus
0.0537
-
 carnitine heart homogenate, pH 8.0, temperature not specified in the publication Mus musculus
0.0562
-
 carnitine mitochondria from heart, pH 8.0, temperature not specified in the publication Mus musculus
0.0664
-
 CoA heart homogenate, pH 8.0, temperature not specified in the publication Mus musculus
0.0888
-
 CoA mitochondria from liver, pH 8.0, temperature not specified in the publication Mus musculus
0.1134
-
 CoA liver homogenate, pH 8.0, temperature not specified in the publication Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-

Organism

Organism UniProt Comment Textmining
Mus musculus P47934
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Mus musculus
-
liver
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + carnitine
-
 Mus musculus CoA + O-acetylcarnitine
-
 r
CoA + O-acetylcarnitine
-
 Mus musculus acetyl-CoA + carnitine
-
 r

General Information

General Information Comment Organism
physiological function in the murine cardiomyocyte cytosol, reverse CrAT activity (producing acetyl-CoA) is higher compared with the liver. The heart displays a lower reverse CrAT Km for CoA compared with the liver. Cytosolic reverse CrAT accounts for 4.6% of total activity in heart tissue and 12.7% in H9C2 cells, while highly purified heart cytosolic fractions show significant CrAT protein levels. Acetyl-CoA carboxylase ACC2-knockout mouse hearts show decreased CrAT protein levels and activity, associated with increased palmitate oxidation and acetyl-CoA/CoA ratio compared with controls. Feeding mice a high-fat diet for 10 weeks increases cardiac CrAT protein levels and activity, associated with a reduced acetyl-CoA/CoA ratio and glucose oxidation Mus musculus