Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.65 extracted from

  • Styles, N.A.; Shonsey, E.M.; Falany, J.L.; Guidry, A.L.; Barnes, S.; Falany, C.N.
    Carboxy-terminal mutations of bile acid CoA N-acyltransferase alter activity and substrate specificity (2016), J. Lipid Res., 57, 1133-1143 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.65

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
BAAT-12AA Trunc mutant enzyme BAAT-12AA Trunc with 12 removed carboxy-terminal amino acids (407-418) Homo sapiens
BAAT-Ex1Del the amino (N)-terminal truncated mutant enzyme BAAT-Ex1Del possess no detectable N-acyltransferase activity Homo sapiens
BAAT-S the mutant BAAT-S, lacking the final two amino acids, shows slightly reduced enzyme activity Homo sapiens
BAAT-SKL mutant BAAT-SKL with a canonical PTS sequence has greater than a 2.5fold increase in N-acyltransferase activity compared with wild type enzyme using cholyl-CoA and taurine as substrates Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Pex5
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 choloyl-CoA mutant enzyme BAAT-S, at pH 8.4 and 37°C Homo sapiens
0.185
-
 choloyl-CoA mutant enzyme BAAT-SKL, at pH 8.4 and 37°C Homo sapiens
0.185
-
 choloyl-CoA wild type enzyme, at pH 8.4 and 37°C Homo sapiens
1.01
-
 taurine mutant enzyme BAAT-SKL, at pH 8.4 and 37°C Homo sapiens
1.77
-
 taurine mutant enzyme BAAT-S, at pH 8.4 and 37°C Homo sapiens
1.86
-
 taurine wild type enzyme, at pH 8.4 and 37°C Homo sapiens
2.14
-
 glycine mutant enzyme BAAT-SKL, at pH 8.4 and 37°C Homo sapiens
3.95
-
 glycine wild type enzyme, at pH 8.4 and 37°C Homo sapiens
4.08
-
 glycine mutant enzyme BAAT-S, at pH 8.4 and 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
peroxisome
-
 Homo sapiens 5777
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
choloyl-CoA + glycine Homo sapiens
-
 CoA + glycocholate
-
 ?
choloyl-CoA + taurine Homo sapiens
-
 CoA + taurocholate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choloyl-CoA + glycine
-
 Homo sapiens CoA + glycocholate
-
 ?
choloyl-CoA + taurine
-
 Homo sapiens CoA + taurocholate
-
 ?

Synonyms

Synonyms Comment Organism
BAAT
-
 Homo sapiens
bile acid CoA:amino acid N-acyltransferase
-
 Homo sapiens
bile acid CoA:N-acyltransferase
-
 Homo sapiens