Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C235A | activity is less than 0.4% of the wild-type activity | Homo sapiens |
C235S | mutation converts the enzyme into an efficient bile acid-CoA thioesterase | Homo sapiens |
D326A | activity is less than 0.4% of the wild-type activity | Homo sapiens |
H362Q | activity is less than 0.4% of the wild-type activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | may play important roles in protection against toxicity by accumulation of unconjugated bile acids and non-esterified very long-chain fatty acids | ? | - |
? | |
additional information | Homo sapiens | may play important roles in protection against toxicity by accumulation of unconjugated bile acids and non-esterified very long-chain fatty acids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
recombinant enzyme | - |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gall bladder | - |
Mus musculus | - |
gall bladder epithelium | - |
Homo sapiens | - |
intestine | - |
Mus musculus | - |
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
pancreas | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.041 | - |
activity with choloyl-CoA anf glycine as substrates | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chenodeoxycholoyl-CoA + glycine | glycine is a slightly better substrate than taurine, thioesterase activity in absence of glycine is about 20% of the conjugation activity | Homo sapiens | CoA + glycochenodeoxycholate | - |
? | |
chenodeoxycholoyl-CoA + taurine | - |
Homo sapiens | CoA + taurochenodeoxycholate | - |
? | |
choloyl-CoA + glycine | glycine is a slightly better substrate than taurine, thioesterase activity in absence of glycine is about 20% of the conjugation activity | Homo sapiens | CoA + glycocholate | - |
? | |
choloyl-CoA + taurine | - |
Homo sapiens | CoA + taurocholate | - |
? | |
additional information | may play important roles in protection against toxicity by accumulation of unconjugated bile acids and non-esterified very long-chain fatty acids | Mus musculus | ? | - |
? | |
additional information | may play important roles in protection against toxicity by accumulation of unconjugated bile acids and non-esterified very long-chain fatty acids | Homo sapiens | ? | - |
? | |
additional information | in addition to its bile acid conjugating activity, the enzyme also possesses thioesterase and glycine conjugating activities with long- and very long-chain acyl-CoAs, at least in vitro. The enzyme can also hydrolyze long- and very-long-chain saturated acyl-CoAs (mainly C16:0-C26:0), the enzyme also conjugates fatty acids to glycine | Homo sapiens | ? | - |
? | |
trihydroxycholestanoyl-CoA + glycine | very low activity | Homo sapiens | CoA + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BACAT | - |
Mus musculus |
BACAT | - |
Homo sapiens |