BRENDA - Enzyme Database show
show all sequences of 2.3.1.65

Conserved residues in the putative catalytic triad of human bile acid coenzyme A:amino acid N-acyltransferase

Sfakianos, M.K.; Wilson, L.; Sakalian, M.; Falany, C.N.; Barnes, S.; J. Biol. Chem. 277, 47270-47275 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene/full-length cDNA is cloned and expressed in Escherichia coli XL1-Blue or BL21
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
C235S
mutant with lower enzyme activity
Homo sapiens
C372A
mutant with low enzyme activity
Homo sapiens
D328A
inactive enzyme
Homo sapiens
H362A
inactive enzyme
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
Homo sapiens
N-ethylmaleimide
10 min, at 0.2 mM: 90% loss of activity, preincubation with cholyl-CoA before NEM-treatment protects
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
x * 50000, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
choloyl-CoA + glycine
Homo sapiens
conjugation of bile acids with glycine or taurine favors their excretion into bile and uptake from portal blood into the liver, it promotes absorption of fat and fat-soluble vitamins in the acidic environment of the small intestine by lowering the pKa of the bile acids and hence maintaining its solubility
CoA + glycocholate
-
-
?
choloyl-CoA + glycine
Homo sapiens
taurine can replace glycine, involved in conjugation of bile acids
CoA + glycocholate
-
-
?
choloyl-CoA + taurine
Homo sapiens
conjugation of bile acids with glycine or taurine favors their excretion into bile and uptake from portal blood into the liver, it promotes absorption of fat and fat-soluble vitamins in the acidic environment of the small intestine by lowering the pKa of the bile acids and hence maintaining its solubility
CoA + taurocholate
-
-
?
choloyl-CoA + taurine
Homo sapiens
taurine can replace glycine, involved in conjugation of bile acids
CoA + taurocholate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
-
Homo sapiens
Reaction
Reaction
Commentary
Organism
choloyl-CoA + glycine = CoA + glycocholate
catalytic mechanism
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
choloyl-CoA + 2-fluoro-beta-alanine
2-fluoro-beta-alanine is an excellent substrate like taurine
487372
Homo sapiens
CoA + 2-fluoro-beta-alanylcholate
-
-
-
?
choloyl-CoA + beta-alanine
beta-alanine is a poor substrate
487372
Homo sapiens
CoA + beta-alanylcholate
-
-
-
?
choloyl-CoA + glycine
-
487372
Homo sapiens
CoA + glycocholate
-
487372
Homo sapiens
?
choloyl-CoA + glycine
conjugation of bile acids with glycine or taurine favors their excretion into bile and uptake from portal blood into the liver, it promotes absorption of fat and fat-soluble vitamins in the acidic environment of the small intestine by lowering the pKa of the bile acids and hence maintaining its solubility
487372
Homo sapiens
CoA + glycocholate
-
-
-
?
choloyl-CoA + glycine
taurine can replace glycine, involved in conjugation of bile acids
487372
Homo sapiens
CoA + glycocholate
-
-
-
?
choloyl-CoA + taurine
taurine is an excellent substrate
487372
Homo sapiens
CoA + taurocholate
-
487372
Homo sapiens
?
choloyl-CoA + taurine
conjugation of bile acids with glycine or taurine favors their excretion into bile and uptake from portal blood into the liver, it promotes absorption of fat and fat-soluble vitamins in the acidic environment of the small intestine by lowering the pKa of the bile acids and hence maintaining its solubility
487372
Homo sapiens
CoA + taurocholate
-
-
-
?
choloyl-CoA + taurine
taurine can replace glycine, involved in conjugation of bile acids
487372
Homo sapiens
CoA + taurocholate
-
-
-
?
additional information
essential catalytic triad consisting of Cys-235, His-362 and Asp-328 with Cys-235 serving as the probable nucleophile and thus the site of covalent attachment of the bile acid molecule
487372
Homo sapiens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 50000, SDS-PAGE
Homo sapiens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
gene/full-length cDNA is cloned and expressed in Escherichia coli XL1-Blue or BL21
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C235S
mutant with lower enzyme activity
Homo sapiens
C372A
mutant with low enzyme activity
Homo sapiens
D328A
inactive enzyme
Homo sapiens
H362A
inactive enzyme
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
Homo sapiens
N-ethylmaleimide
10 min, at 0.2 mM: 90% loss of activity, preincubation with cholyl-CoA before NEM-treatment protects
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
x * 50000, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
choloyl-CoA + glycine
Homo sapiens
conjugation of bile acids with glycine or taurine favors their excretion into bile and uptake from portal blood into the liver, it promotes absorption of fat and fat-soluble vitamins in the acidic environment of the small intestine by lowering the pKa of the bile acids and hence maintaining its solubility
CoA + glycocholate
-
-
?
choloyl-CoA + glycine
Homo sapiens
taurine can replace glycine, involved in conjugation of bile acids
CoA + glycocholate
-
-
?
choloyl-CoA + taurine
Homo sapiens
conjugation of bile acids with glycine or taurine favors their excretion into bile and uptake from portal blood into the liver, it promotes absorption of fat and fat-soluble vitamins in the acidic environment of the small intestine by lowering the pKa of the bile acids and hence maintaining its solubility
CoA + taurocholate
-
-
?
choloyl-CoA + taurine
Homo sapiens
taurine can replace glycine, involved in conjugation of bile acids
CoA + taurocholate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
choloyl-CoA + 2-fluoro-beta-alanine
2-fluoro-beta-alanine is an excellent substrate like taurine
487372
Homo sapiens
CoA + 2-fluoro-beta-alanylcholate
-
-
-
?
choloyl-CoA + beta-alanine
beta-alanine is a poor substrate
487372
Homo sapiens
CoA + beta-alanylcholate
-
-
-
?
choloyl-CoA + glycine
-
487372
Homo sapiens
CoA + glycocholate
-
487372
Homo sapiens
?
choloyl-CoA + glycine
conjugation of bile acids with glycine or taurine favors their excretion into bile and uptake from portal blood into the liver, it promotes absorption of fat and fat-soluble vitamins in the acidic environment of the small intestine by lowering the pKa of the bile acids and hence maintaining its solubility
487372
Homo sapiens
CoA + glycocholate
-
-
-
?
choloyl-CoA + glycine
taurine can replace glycine, involved in conjugation of bile acids
487372
Homo sapiens
CoA + glycocholate
-
-
-
?
choloyl-CoA + taurine
taurine is an excellent substrate
487372
Homo sapiens
CoA + taurocholate
-
487372
Homo sapiens
?
choloyl-CoA + taurine
conjugation of bile acids with glycine or taurine favors their excretion into bile and uptake from portal blood into the liver, it promotes absorption of fat and fat-soluble vitamins in the acidic environment of the small intestine by lowering the pKa of the bile acids and hence maintaining its solubility
487372
Homo sapiens
CoA + taurocholate
-
-
-
?
choloyl-CoA + taurine
taurine can replace glycine, involved in conjugation of bile acids
487372
Homo sapiens
CoA + taurocholate
-
-
-
?
additional information
essential catalytic triad consisting of Cys-235, His-362 and Asp-328 with Cys-235 serving as the probable nucleophile and thus the site of covalent attachment of the bile acid molecule
487372
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 50000, SDS-PAGE
Homo sapiens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
Other publictions for EC 2.3.1.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
702070
Buch
Localization of peroxisomal ma ...
Homo sapiens
Biochem. Biophys. Res. Commun.
388
355-359
2009
-
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1
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1
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2
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1
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1
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1
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706769
Chen
Retinoid X receptor alpha part ...
Rattus norvegicus
Shock
32
164-171
2009
-
-
-
-
-
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1
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2
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5
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1
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1
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5
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1
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1
1
1
1
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-
688232
Shonsey
Inactivation of human liver bi ...
Homo sapiens
J. Lipid Res.
49
282-294
2008
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1
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1
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1
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-
673916
Pellicoro
Human and rat bile acid-CoA:am ...
Homo sapiens, Rattus norvegicus
Hepatology
45
340-348
2007
-
-
1
-
-
-
-
-
7
-
-
2
-
4
-
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6
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2
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1
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7
-
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2
-
-
-
-
-
6
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686362
Tougou
Genetic polymorphism of bile a ...
Homo sapiens
Drug Metab. Pharmacokinet.
22
125-128
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
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1
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1
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1
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-
-
-
-
-
-
-
-
-
690075
Chen
Role of retinoid-X receptor-al ...
Rattus norvegicus
Shock
28
65-70
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
3
-
-
1
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1
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3
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
675804
Shonsey
Bile acid coenzyme A: amino ac ...
Homo sapiens, Rattus norvegicus
Methods Enzymol.
400
374-394
2005
-
-
2
-
-
-
-
4
-
-
-
-
-
2
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1
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-
2
1
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4
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2
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4
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1
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2
1
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4
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-
-
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-
-
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-
-
-
-
659246
O'Byrne
The Human bile acid-CoA:amino ...
Homo sapiens, Mus musculus
J. Biol. Chem.
278
34237-34244
2003
-
-
1
-
4
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-
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2
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2
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3
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1
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8
1
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8
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1
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4
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2
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2
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1
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8
1
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8
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-
659664
He
Rat liver: Expression, charact ...
Rattus norvegicus
J. Lipid Res.
44
2242-2249
2003
-
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1
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2
1
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5
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2
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2
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1
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1
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2
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2
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487372
Sfakianos
Conserved residues in the puta ...
Homo sapiens
J. Biol. Chem.
277
47270-47275
2002
-
-
1
-
4
-
2
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1
4
-
3
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1
1
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1
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9
1
1
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1
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4
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2
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1
4
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1
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1
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9
1
1
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487373
Falany
Cloning, expression, and chrom ...
Mus musculus
J. Lipid Res.
38
1139-1148
1997
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1
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1
1
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2
1
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1
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1
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2
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4
1
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1
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1
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1
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1
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1
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2
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4
1
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487371
Ide
Dietary modifications of the b ...
Rattus norvegicus
Br. J. Nutr.
72
93-100
1994
1
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6
1
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2
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3
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3
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1
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1
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6
1
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3
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4
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1
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487366
Czuba
Structural characterization of ...
Bos taurus
J. Biol. Chem.
261
6260-6263
1986
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1
2
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1
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5
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1
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5
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487367
Kimura
Purification and characterizat ...
Homo sapiens
Hoppe-Seyler's Z. Physiol. Chem.
364
637-645
1983
3
-
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-
1
10
4
-
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3
4
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1
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1
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1
2
1
17
1
1
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-
-
3
1
-
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-
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-
3
-
-
-
-
-
1
-
10
-
4
-
-
3
4
-
-
-
1
-
1
2
1
17
1
1
-
-
-
3
1
-
-
-
-
-
-
-
-
487369
Czuba
Identification of a unique mam ...
Canis lupus familiaris
Biochim. Biophys. Acta
665
612-614
1981
-
-
-
-
-
1
-
-
1
-
1
1
-
1
-
-
1
-
-
1
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
-
-
1
-
1
1
-
-
-
1
-
1
1
-
3
-
-
-
-
-
-
-
-
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-
-
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-
487365
Czuba
Kinetic characterization of ch ...
Bos taurus
J. Biol. Chem.
255
5296-5299
1980
-
-
-
-
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1
7
4
-
-
-
2
-
2
-
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1
1
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2
1
-
10
-
1
-
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1
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1
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-
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-
-
-
-
-
1
-
7
1
4
-
-
-
2
-
-
-
1
-
2
1
-
10
-
1
-
-
1
-
-
-
-
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-
487364
Vessey
The co-purification and common ...
Bos taurus
J. Biol. Chem.
254
2059-2063
1979
-
-
-
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-
2
-
1
1
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2
2
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1
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1
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1
2
1
4
1
-
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4
1
1
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2
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1
1
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2
2
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1
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1
2
1
4
1
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4
1
1
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Killenberg
Purification and characterizat ...
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1978
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1
5
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1
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2
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1
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3
3
2
15
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2
1
2
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5
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1
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1
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3
3
2
15
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1
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2
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2
1
2
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487370
Poley
Bile acids in infants and chil ...
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1964
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