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Literature summary for 2.3.1.65 extracted from
- Purification and characterization of bile acid-CoA:amino acid N-acyltransferase from rat liver (1978), J. Biol. Chem., 253, 1005-1010.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Rattus norvegicus | |
| 0.8 | - |
taurine | + 0.05 mM deoxycholoyl-CoA | Rattus norvegicus | |
| 0.8 | - |
aminomethanesulfonate | + 0.05 mM deoxycholoyl-CoA | Rattus norvegicus | |
| 31 | - |
glycine | + 0.05 mM deoxycholoyl-CoA | Rattus norvegicus | |
| 175 | - |
beta-Alanine | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choloyl-CoA + taurine | Rattus norvegicus | taurine conjugates predominate in rat bile at normal hepatocellular concentrations of glycine and taurine | CoA + taurocholate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, 200fold | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| 1.99 | - |
taurine | Rattus norvegicus |
| 3.88 | - |
glycine | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, several weeks, stable | Rattus norvegicus |
| 0° to 20°C, several h, stable | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chenodeoxycholoyl-CoA + glycine | - |
Rattus norvegicus | CoA + glycochenodeoxycholate | - |
? | |
| chenodeoxycholoyl-CoA + taurine | - |
Rattus norvegicus | CoA + taurochenodeoxycholate | - |
? | |
| choloyl-CoA + beta-alanine | - |
Rattus norvegicus | CoA + beta-alanylcholate | - |
? | |
| choloyl-CoA + glycine | - |
Rattus norvegicus | CoA + glycocholate | - |
? | |
| choloyl-CoA + taurine | preferred substrate | Rattus norvegicus | CoA + taurocholate | - |
? | |
| choloyl-CoA + taurine | taurine conjugates predominate in rat bile at normal hepatocellular concentrations of glycine and taurine | Rattus norvegicus | CoA + taurocholate | - |
? | |
| deoxycholoyl-CoA + aminomethanesulfonate | - |
Rattus norvegicus | CoA + aminomethanesulfonyldeoxycholate | - |
? | |
| deoxycholoyl-CoA + glycine | - |
Rattus norvegicus | CoA + glycodeoxycholate | - |
? | |
| deoxycholoyl-CoA + taurine | - |
Rattus norvegicus | CoA + taurodeoxycholate | - |
? | |
| lithocholoyl-CoA + glycine | - |
Rattus norvegicus | CoA + glycolithocholate | - |
? | |
| lithocholoyl-CoA + taurine | - |
Rattus norvegicus | CoA + taurolithocholate | - |
? | |
| additional information | not: D-alanine, L-arginine, L-lysine, 2-hydroxyethanesulfonate, 2-aminoethanesulfonate, 1-aminoethanephosphonate and 2-aminoethanephosphonate, 3-aminopropanesulfonate | Rattus norvegicus | ? | - |
? | |
| additional information | not as acyl-CoA donors: acetyl-CoA, phenylacetyl-CoA and benzoyl-CoA | Rattus norvegicus | ? | - |
? | |
| additional information | not as acyl-CoA donors: succinoyl-CoA, palmitoyl-CoA | Rattus norvegicus | ? | - |
? | |
| additional information | not: L-alpha-alanine, L-glutamine, L-ornithine | Rattus norvegicus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable below | Rattus norvegicus |
| 50 | - |
inactivation above | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 7.2 | Rattus norvegicus |
| 7.8 | 8 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | about 70% of maximal activity at pH 6.0, about half-maximal activity at pH 9.0, inactivation rate is similar for both taurine and glycine | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
phosphate buffer, t1/2: 2 h | Rattus norvegicus |
| 9 | - |
Tris-buffer, t1/2: 3 h | Rattus norvegicus |
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