BRENDA - Enzyme Database
show all sequences of 2.3.1.64

A new class of N-hydroxycinnamoyltransferases. Purification, cloning, and expression of barley agmatine coumaroyltransferase (EC 2.3.1.64)

Burhenne, K.; Kristensen, B.K.; Rasmussen, S.K.; J. Biol. Chem. 278, 13919-13927 (2003)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
1-mercapto-2,3-propanediol
1 mM is required for optimal activity
Hordeum vulgare
2-mercaptoethanol
1 mM is required for optimal activity
Hordeum vulgare
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Hordeum vulgare
Inhibitors
Inhibitors
Commentary
Organism
Structure
CuSO4
10 mM, 85% inhibition
Hordeum vulgare
dithiothreitol
30% inhibition
Hordeum vulgare
MnCl2
10 mM, 29% inhibition
Hordeum vulgare
ZnSO4
10 mM, 99% inhibition
Hordeum vulgare
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
4-Coumaroyl-CoA
isoenzyme 1
Hordeum vulgare
0.0016
-
4-Coumaroyl-CoA
isoenzyme 3
Hordeum vulgare
0.0017
-
feruloyl-CoA
isoenzyme 3
Hordeum vulgare
0.0021
-
4-Coumaroyl-CoA
isoenzyme 2; recombinant enzyme
Hordeum vulgare
0.0041
-
feruloyl-CoA
isoenzyme 2
Hordeum vulgare
0.0043
-
caffeoyl-CoA
isoenzyme 2; recombinant enzyme
Hordeum vulgare
0.0052
-
agmatine
isoenzyme 1; isoenzyme 3
Hordeum vulgare
0.0053
-
caffeoyl-CoA
isoenzyme 3
Hordeum vulgare
0.0058
-
caffeoyl-CoA
isoenzyme 1
Hordeum vulgare
0.0061
-
feruloyl-CoA
isoenzyme 1
Hordeum vulgare
0.0065
-
agmatine
isoenzyme 2
Hordeum vulgare
0.0066
-
feruloyl-CoA
recombinant enzyme
Hordeum vulgare
0.0081
-
agmatine
recombinant enzyme
Hordeum vulgare
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
-
Hordeum vulgare
48000
-
1 * 48000, SDS-PAGE
Hordeum vulgare
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hordeum vulgare
A9ZPJ6
isoenzymes 1-3
-
Purification (Commentary)
Commentary
Organism
Blue Sepharose, t-butyl-Sepharose, Resource Q, Superose 12 HR
Hordeum vulgare
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.78
-
-
Hordeum vulgare
11.34
-
recombinant enzyme
Hordeum vulgare
Storage Stability
Storage Stability
Organism
0°C, 100 mM Tris, pH 7.5, 1 mM EDTA, 10 mM 2-mercaptoethanol, 50 mM KCl
Hordeum vulgare
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + agmatine
-
487363
Hordeum vulgare
CoA + 4-coumaroylagmatine
-
-
-
-
caffeoyl-CoA + agmatine
-
487363
Hordeum vulgare
CoA + caffeoylagmatine
-
-
-
-
feruloyl-CoA + agmatine
-
487363
Hordeum vulgare
CoA + feruloyl agmatine
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 48000, SDS-PAGE
Hordeum vulgare
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Hordeum vulgare
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
50
-
Hordeum vulgare
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Hordeum vulgare
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.9
8.1
half-maximal activity at pH 6.9 and pH 8.1
Hordeum vulgare
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
1-mercapto-2,3-propanediol
1 mM is required for optimal activity
Hordeum vulgare
2-mercaptoethanol
1 mM is required for optimal activity
Hordeum vulgare
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Hordeum vulgare
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuSO4
10 mM, 85% inhibition
Hordeum vulgare
dithiothreitol
30% inhibition
Hordeum vulgare
MnCl2
10 mM, 29% inhibition
Hordeum vulgare
ZnSO4
10 mM, 99% inhibition
Hordeum vulgare
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
4-Coumaroyl-CoA
isoenzyme 1
Hordeum vulgare
0.0016
-
4-Coumaroyl-CoA
isoenzyme 3
Hordeum vulgare
0.0017
-
feruloyl-CoA
isoenzyme 3
Hordeum vulgare
0.0021
-
4-Coumaroyl-CoA
isoenzyme 2; recombinant enzyme
Hordeum vulgare
0.0041
-
feruloyl-CoA
isoenzyme 2
Hordeum vulgare
0.0043
-
caffeoyl-CoA
isoenzyme 2; recombinant enzyme
Hordeum vulgare
0.0052
-
agmatine
isoenzyme 1; isoenzyme 3
Hordeum vulgare
0.0053
-
caffeoyl-CoA
isoenzyme 3
Hordeum vulgare
0.0058
-
caffeoyl-CoA
isoenzyme 1
Hordeum vulgare
0.0061
-
feruloyl-CoA
isoenzyme 1
Hordeum vulgare
0.0065
-
agmatine
isoenzyme 2
Hordeum vulgare
0.0066
-
feruloyl-CoA
recombinant enzyme
Hordeum vulgare
0.0081
-
agmatine
recombinant enzyme
Hordeum vulgare
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
-
Hordeum vulgare
48000
-
1 * 48000, SDS-PAGE
Hordeum vulgare
Purification (Commentary) (protein specific)
Commentary
Organism
Blue Sepharose, t-butyl-Sepharose, Resource Q, Superose 12 HR
Hordeum vulgare
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.78
-
-
Hordeum vulgare
11.34
-
recombinant enzyme
Hordeum vulgare
Storage Stability (protein specific)
Storage Stability
Organism
0°C, 100 mM Tris, pH 7.5, 1 mM EDTA, 10 mM 2-mercaptoethanol, 50 mM KCl
Hordeum vulgare
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + agmatine
-
487363
Hordeum vulgare
CoA + 4-coumaroylagmatine
-
-
-
-
caffeoyl-CoA + agmatine
-
487363
Hordeum vulgare
CoA + caffeoylagmatine
-
-
-
-
feruloyl-CoA + agmatine
-
487363
Hordeum vulgare
CoA + feruloyl agmatine
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 48000, SDS-PAGE
Hordeum vulgare
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Hordeum vulgare
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
50
-
Hordeum vulgare
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Hordeum vulgare
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.9
8.1
half-maximal activity at pH 6.9 and pH 8.1
Hordeum vulgare
Other publictions for EC 2.3.1.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721048
Muroi
Acquired immunity of transgeni ...
Arabidopsis thaliana
Sci. Rep.
2
689
2012
-
1
1
-
1
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706386
Muroi
Accumulation of hydroxycinnami ...
Arabidopsis thaliana
Planta
230
517-527
2009
-
-
1
-
-
-
4
-
-
-
1
4
-
5
-
-
1
-
-
1
8
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
1
4
-
-
-
1
-
1
8
-
4
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
686954
Nomura
Chromosome arm location of the ...
Hordeum vulgare, Triticum aestivum
Genes Genet. Syst.
82
455-464
2007
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487363
Burhenne
A new class of N-hydroxycinnam ...
Hordeum vulgare
J. Biol. Chem.
278
13919-13927
2003
2
-
1
-
-
-
4
13
-
-
2
-
-
5
-
-
1
-
-
1
2
1
3
1
1
1
-
-
1
1
-
-
-
-
-
2
-
1
-
-
-
-
-
4
-
13
-
-
2
-
-
-
-
1
-
1
2
1
3
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
487361
Bird
-
Agmatine coumaroyltransferase ...
Hordeum vulgare
Methods Enzymol.
94
344-347
1983
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
1
1
1
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
487362
Bird
-
Agmatine coumaroyltransferase ...
Hordeum vulgare
Phytochemistry
22
2401-2403
1983
-
-
-
-
-
1
-
6
1
-
1
-
-
1
-
-
1
-
-
1
1
-
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
6
1
-
1
-
-
-
-
1
-
1
1
-
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
487360
Bird
-
The biosynthesis of coumaroyla ...
Hordeum vulgare
Phytochemistry
20
2345-2346
1981
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-