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Literature summary for 2.3.1.64 extracted from

  • Bird, C.R.; Smith, T.A.
    Agmatine coumaroyltransferase (barley seedlings) (1983), Methods Enzymol., 94, 344-347.
No PubMed abstract available
Search for more literature for 2.3.1.64

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00074
-
 4-coumaroyl-CoA
-
 Hordeum vulgare
0.012
-
 agmatine
-
 Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Biogel A, Sepharose 4B-agmatine Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
seedling shoots, not seeds Hordeum vulgare
-
seedling maximal activity 3-4 days after germination at 22°C in the dark Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.393
-
-
 Hordeum vulgare

Storage Stability

Storage Stability Organism
-40°C, concentrated preparation at least 1 month, negligible loss of activity Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + agmatine no activity with putrescine, spermidine, spermine, cadaverine, arginine, homoarginine Hordeum vulgare CoA + 4-coumaroylagmatine
-
 ?
caffeoyl-CoA + agmatine
-
 Hordeum vulgare CoA + caffeoylagmatine
-
 ?
feruloyl-CoA + agmatine
-
 Hordeum vulgare CoA + feruloyl agmatine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Hordeum vulgare

pH Range

pH Minimum pH Maximum Comment Organism
7 8 approx. half-maximal activity at pH 7.0 and 8.0 Hordeum vulgare