BRENDA - Enzyme Database
show all sequences of 2.3.1.64

Agmatine coumaroyltransferase (barley seedlings)

Bird, C.R.; Smith, T.A.; Methods Enzymol. 94, 344-347 (1983)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00074
-
4-Coumaroyl-CoA
-
Hordeum vulgare
0.012
-
agmatine
-
Hordeum vulgare
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
gel filtration
Hordeum vulgare
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hordeum vulgare
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, Biogel A, Sepharose 4B-agmatine
Hordeum vulgare
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
maximal activity 3-4 days after germination at 22°C in the dark; shoots, not seeds
Hordeum vulgare
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.393
-
-
Hordeum vulgare
Storage Stability
Storage Stability
Organism
-40°C, concentrated preparation at least 1 month, negligible loss of activity
Hordeum vulgare
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + agmatine
no activity with putrescine, spermidine, spermine, cadaverine, arginine, homoarginine
487361
Hordeum vulgare
CoA + 4-coumaroylagmatine
-
487361
Hordeum vulgare
?
caffeoyl-CoA + agmatine
-
487361
Hordeum vulgare
CoA + caffeoylagmatine
-
487361
Hordeum vulgare
?
feruloyl-CoA + agmatine
-
487361
Hordeum vulgare
CoA + feruloyl agmatine
-
487361
Hordeum vulgare
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Hordeum vulgare
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
8
approx. half-maximal activity at pH 7.0 and 8.0
Hordeum vulgare
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00074
-
4-Coumaroyl-CoA
-
Hordeum vulgare
0.012
-
agmatine
-
Hordeum vulgare
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
gel filtration
Hordeum vulgare
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, Biogel A, Sepharose 4B-agmatine
Hordeum vulgare
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
maximal activity 3-4 days after germination at 22°C in the dark; shoots, not seeds
Hordeum vulgare
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.393
-
-
Hordeum vulgare
Storage Stability (protein specific)
Storage Stability
Organism
-40°C, concentrated preparation at least 1 month, negligible loss of activity
Hordeum vulgare
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + agmatine
no activity with putrescine, spermidine, spermine, cadaverine, arginine, homoarginine
487361
Hordeum vulgare
CoA + 4-coumaroylagmatine
-
487361
Hordeum vulgare
?
caffeoyl-CoA + agmatine
-
487361
Hordeum vulgare
CoA + caffeoylagmatine
-
487361
Hordeum vulgare
?
feruloyl-CoA + agmatine
-
487361
Hordeum vulgare
CoA + feruloyl agmatine
-
487361
Hordeum vulgare
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Hordeum vulgare
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
8
approx. half-maximal activity at pH 7.0 and 8.0
Hordeum vulgare
Other publictions for EC 2.3.1.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721048
Muroi
Acquired immunity of transgeni ...
Arabidopsis thaliana
Sci. Rep.
2
689
2012
-
1
1
-
1
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
1
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1
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-
-
-
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-
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-
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-
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-
706386
Muroi
Accumulation of hydroxycinnami ...
Arabidopsis thaliana
Planta
230
517-527
2009
-
-
1
-
-
-
4
-
-
-
1
4
-
5
-
-
1
-
-
1
8
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
1
4
-
-
-
1
-
1
8
-
4
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
686954
Nomura
Chromosome arm location of the ...
Hordeum vulgare, Triticum aestivum
Genes Genet. Syst.
82
455-464
2007
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
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2
-
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-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487363
Burhenne
A new class of N-hydroxycinnam ...
Hordeum vulgare
J. Biol. Chem.
278
13919-13927
2003
2
-
1
-
-
-
4
13
-
-
2
-
-
5
-
-
1
-
-
1
2
1
3
1
1
1
-
-
1
1
-
-
-
-
-
2
-
1
-
-
-
-
-
4
-
13
-
-
2
-
-
-
-
1
-
1
2
1
3
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
487361
Bird
-
Agmatine coumaroyltransferase ...
Hordeum vulgare
Methods Enzymol.
94
344-347
1983
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
1
1
1
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
487362
Bird
-
Agmatine coumaroyltransferase ...
Hordeum vulgare
Phytochemistry
22
2401-2403
1983
-
-
-
-
-
1
-
6
1
-
1
-
-
1
-
-
1
-
-
1
1
-
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
6
1
-
1
-
-
-
-
1
-
1
1
-
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
487360
Bird
-
The biosynthesis of coumaroyla ...
Hordeum vulgare
Phytochemistry
20
2345-2346
1981
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-