Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.6 extracted from

  • Habert, E.; Birman, S.; Mallet, J.
    High-level synthesis and fate of acetylcholine in baculovirus-infected cells: characterization and purification of recombinant rat choline acetyltransferase (1992), J. Neurochem., 58, 1447-1453.
    View publication on PubMed
Search for more literature for 2.3.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Spodoptera frugiperda Sf9 cells Rattus norvegicus
expression in Spodoptera frugiperda Sf9 cells using a baculovirus expression system Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.221
-
 acetyl-CoA
-
 Rattus norvegicus
0.299
-
 choline
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68500
-
 1 * 68500, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein rat choline acetyltransferase is phosphorylated in Sf9 cells Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.19
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + choline
-
 Rattus norvegicus acetylcholine + CoA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 68500, SDS-PAGE Rattus norvegicus