Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
in complex with spermidine and CoA, sitting drop vapor diffusion method, using 0.1 M Ca-acetate, 0.05 M Na-cacodylate pH 6.5, and 9% (w/v) PEG8000 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E33Q | the mutant shows greatly reduced enzyme activity compared to the wild type enzyme | Escherichia coli |
E34Q | inactive | Escherichia coli |
E37Q | inactive | Escherichia coli |
E41Q | inactive | Escherichia coli |
E55Q | the mutant shows greatly reduced enzyme activity compared to the wild type enzyme | Escherichia coli |
E71Q | inactive | Escherichia coli |
E74Q | the mutant shows greatly reduced enzyme activity compared to the wild type enzyme | Escherichia coli |
E83Q | the mutant shows greatly reduced enzyme activity compared to the wild type enzyme | Escherichia coli |
O85E | the mutant shows greatly reduced enzyme activity compared to the wild type enzyme | Escherichia coli |
Q53A | the mutant shows greatly reduced enzyme activity compared to the wild type enzyme | Escherichia coli |
R56A | inactive | Escherichia coli |
W31A | inactive | Escherichia coli |
Y133F | the mutant shows increased enzyme activity compared to the wild type enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + spermidine | Escherichia coli | - |
CoA + N1-acetylspermidine | - |
? | |
acetyl-CoA + spermidine | Escherichia coli LY180 | - |
CoA + N1-acetylspermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | A0A0M3KKU5 | - |
- |
Escherichia coli LY180 | A0A0M3KKU5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + spermidine | - |
Escherichia coli | CoA + N1-acetylspermidine | - |
? | |
acetyl-CoA + spermidine | - |
Escherichia coli LY180 | CoA + N1-acetylspermidine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | X-ray crystallography, the dodecamer is organized as a hexamer of dimers | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
SAT | - |
Escherichia coli |
spermidine acetyltransferase | - |
Escherichia coli |
spermidine/spermine N1-acetyltransferase | - |
Escherichia coli |
SSAT | - |
Escherichia coli |