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Literature summary for 2.3.1.57 extracted from
- Structural and functional evidence for Bacillus subtilis PaiA as a novel N1-spermidine/spermine acetyltransferase (2005), J. Biol. Chem., 280, 40328-40336.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus subtilis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, crystal structure of PaiA in complex with CoA at 1.9A resolution, crystals belong to space group C222(1), with cell parameters of alpha = 39.9 A, beta = 135.8 A and gamma = 132.4 A | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
acetyl-CoA | pH 9 | Bacillus subtilis |  |
| 0.076 | - |
spermine | pH 9 | Bacillus subtilis | |
| 0.295 | - |
N1-acetylspermine | pH 9 | Bacillus subtilis | |
| 0.323 | - |
spermidine | pH 9 | Bacillus subtilis | |
| 0.41 | - |
aminopropylcadaverine | pH 9 | Bacillus subtilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + aminopropylcadaverine | - |
Bacillus subtilis | ? | - |
? | |
| acetyl-CoA + N1-acetylspermine | - |
Bacillus subtilis | CoA + N1,N12-diacetylspermine | - |
? | |
| acetyl-CoA + spermidine | - |
Bacillus subtilis | CoA + N1-acetylspermidine | - |
? | |
| acetyl-CoA + spermine | - |
Bacillus subtilis | CoA + N1-acetylspermine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| N1-spermidine/spermine acetyltransferase | - |
Bacillus subtilis |
| PaiA | - |
Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.087 | - |
spermidine | pH 9 | Bacillus subtilis | |
| 0.1 | - |
aminopropylcadaverine | pH 9 | Bacillus subtilis | |
| 0.12 | - |
N1-acetylspermine | pH 9 | Bacillus subtilis | |
| 0.32 | - |
spermine | pH 9 | Bacillus subtilis | |
| 0.365 | - |
acetyl-CoA | pH 9, cosubstrate: spermine | Bacillus subtilis | |
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