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Literature summary for 2.3.1.57 extracted from
- Effect of expression of human spermidine/spermine N1-acetyltransferase in Escherichia coli (1995), Biochemistry, 34, 2701-2709.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | N1,N12-bis(ethyl)spermine is a potent inducer of enzyme in human tumor cells | Homo sapiens | |
| additional information | enzyme is induced by isopropyl beta-D-thiogalactopyranoside | Homo sapiens |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | induction of enzyme may be critical for the inhibition of tumor cell growth | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and expression of cDNA encoding enzyme in Escherichia coli DH5alpha leads to a significant reduction in the cell growth rate | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
15-deoxyspergualin | - |
Homo sapiens |  |
| 0.025 | - |
spermine | - |
Homo sapiens | |
| 0.06 | - |
spermidine | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | recombinant enzyme, expressed in Escherichia coli DH5alpha, is present at a level of about 2% of the soluble protein | Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
x * 21000 + x * 23000, two bands, enzyme is probably a tetramer, recombinant enzyme, SDS-PAGE | Homo sapiens |
| 23000 | - |
x * 21000 + x * 23000, two bands, enzyme is probably a tetramer, recombinant enzyme, SDS-PAGE | Homo sapiens |
| 75000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | acetylation is a physiological response to convert excess polyamines to a physiologically inert form which is readily excreted | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
recombinant enzyme, expressed in Escherichia coli DH5alpha | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | no posttranslational modification specific to eukaryotes is needed for enzyme activity | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 62fold purification of recombinant enzyme, expressed in Escherichia coli DH5alpha | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
| 45 | - |
spermidine | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, several weeks, stable | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + (S)-2-[(3-aminopropyl)amino]ethylphosphoric acid | WR-2721, about 10% of the rate with spermidine | Homo sapiens | CoA + N-acetyl-(S)-2-[(3-aminopropyl)amino]ethylphosphoric acid | - |
? | |
| acetyl-CoA + (S)-2-[(3-aminopropyl)amino]propylphosphoric acid | WR-44923, about 10% of the rate with spermidine | Homo sapiens | CoA + N-acetyl-(S)-2-[(3-aminopropyl)amino]propylphosphoric acid | - |
? | |
| acetyl-CoA + 15-deoxyspergualin | antitumor and immunosuppressive agent 15-deoxyspergualin, about 18% of the rate with spermidine | Homo sapiens | ? | - |
? | |
| acetyl-CoA + 2-[(aminopropyl)amino]ethanethiol | radioprotective drug WR-1065, lower affinity than for spermidine, about 10% of the rate with spermidine | Homo sapiens | CoA + N-acetyl-2-[(aminopropyl)amino]ethanethiol | - |
? | |
| acetyl-CoA + N-(n-butyl)-1,3-diaminopropane | weak substrate, lower affinity than for spermidine, 1.3% of the rate with spermidine | Homo sapiens | CoA + N1-acetyl-N3-(n-butyl)-1,3-diaminopropane | - |
? | |
| acetyl-CoA + spermidine | - |
Homo sapiens | CoA + N1-acetylspermidine | product is exclusively N1-acetylspermidine | ? | |
| acetyl-CoA + spermine | about 40% of the rate with spermidine | Homo sapiens | CoA + N1-acetylspermine | - |
? | |
| additional information | not: 1-methylspermidine, WR-2822 | Homo sapiens | ? | - |
? | |
| additional information | enzyme requires a substrate with the structure H2N(CH2)3NHR and acetylates the primary amino group | Homo sapiens | ? | - |
? | |
| additional information | not: putrescine | Homo sapiens | ? | - |
? | |
| additional information | acetylation is a physiological response to convert excess polyamines to a physiologically inert form which is readily excreted | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | x * 21000 + x * 23000, two bands, enzyme is probably a tetramer, recombinant enzyme, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
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