Cloned (Comment) | Organism |
---|---|
genes BAT1.13 and BAT1.5, cloning of two cDNAs encoding microsomal isozymes from an immature embryo library, sequence comparisons, functional expression of the two rapeseed microsomal LPAAT isozymes in Arabidopsis thaliana, using Agrobacterium tumefaciens strain C58C1 transfection, leading to enhanced oil content and seed weight, tissue localization study, overview. Complementationof a thermosensitive Escherichia coli strain JC201 by expression of Bat1.5 and BAT1.13, overview | Brassica napus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | LPAAT is located in the cytoplasmic endomembrane compartment, two microsomal LPAAT isozymes are identified. Diversity of LPAAT microsomal isozymes in rapeseed, overview | Brassica napus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Brassica napus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Brassica napus | i.e. lysophosphatidic acid | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | Q9XFW4 | BAT1.13 and BAT1.5 genes encoding two microsomal LPAAT isozymes | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | BAT1.13 | Brassica napus | - |
leaf | BAT1.13 | Brassica napus | - |
additional information | differences in the expression of the two genes emcoding the two microsomal LPAAT isozymes, one is detected in all rapeseed tissues and during silique and seed development, whereas the expression of the second gene is restricted predominantly to siliques and developing seeds | Brassica napus | - |
root | BAT1.13, expression at the tip of primary and secondary roots and in mesophyll/cortex tissue behind the elongation zone and in the vascular tissue | Brassica napus | - |
seed | developing, BAT1.5 , and BAT1.13 | Brassica napus | - |
silique | young, BAT1.5 , and BAT1.13 | Brassica napus | - |
stem | BAT1.13 | Brassica napus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | i.e. lysophosphatidic acid | Brassica napus | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? | |
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | i.e. lysophosphatidic acid | Brassica napus | CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? |
Synonyms | Comment | Organism |
---|---|---|
LPAAT | - |
Brassica napus |
lysophosphatidic acid acyltransferase | - |
Brassica napus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Brassica napus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Brassica napus |
General Information | Comment | Organism |
---|---|---|
metabolism | LPAAT is a key intermediate in the biosynthesis of membrane phospholipids in all tissues and storage lipids in developing seeds | Brassica napus |
physiological function | LPAAT plays an essential role in the synthesis of phosphatidic acid | Brassica napus |