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Literature summary for 2.3.1.50 extracted from

  • Perry, D.K.
    Serine palmitoyltransferase: role in apoptotic de novo ceramide synthesis and other stress responses (2002), Biochim. Biophys. Acta, 1585, 146-152.
    View publication on PubMed
Search for more literature for 2.3.1.50

Activating Compound

Activating Compound Comment Organism Structure
etoposide enhanced activity due to chemotherapy with epotoside as therapeutic agent in Molt-4 leukemic cells Homo sapiens
fenretinide up-regulates enzyme activity in neuroblastoma cells Homo sapiens
additional information enzyme activity is enhanced under stress and in apoptosis, e.g. in pancreatic cells in a model for diabetes, in case of angiotensin II type receptor occupancy Mus musculus
additional information enzyme activity is enhanced under stress and in apoptosis, e.g. in pancreatic cells in a model for diabetes, in case of angiotensin II type receptor occupancy Homo sapiens
retinoic acid up-regulates enzyme activity in embryonic carcinoma cell ine PCC7-Mz1 Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
genes LCB1 and LCB2, overexpression Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C133V reduced activity Homo sapiens
C133Y reduced activity Homo sapiens
V144D reduced activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
beta-chloro-L-alanine
-
 Homo sapiens
beta-chloro-L-alanine
-
 Mus musculus
beta-chloro-L-alanine
-
 Saccharomyces cerevisiae
L-Cycloserine
-
 Homo sapiens
L-Cycloserine
-
 Mus musculus
L-Cycloserine
-
 Saccharomyces cerevisiae
myriocin
-
 Homo sapiens
myriocin
-
 Mus musculus
myriocin
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum cytosolic side of the membrane Homo sapiens 5783
-
endoplasmic reticulum cytosolic side of the membrane Saccharomyces cerevisiae 5783
-
microsome
-
 Homo sapiens
-
-
microsome
-
 Saccharomyces cerevisiae
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + L-serine Homo sapiens involved in cellular stress response CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine Saccharomyces cerevisiae involved in cellular stress response CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine Mus musculus first step of biosynthesis of sphingolipid bases CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine Homo sapiens first step of biosynthesis of sphingolipid bases CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine Saccharomyces cerevisiae first step of biosynthesis of sphingolipid bases CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine Homo sapiens rate-limiting enzyme in synthesis of sphingolipids CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine Saccharomyces cerevisiae rate-limiting enzyme in synthesis of sphingolipids CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine Homo sapiens initial step of de novo ceramide biosynthesis CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine Saccharomyces cerevisiae initial step of de novo ceramide biosynthesis CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
Saccharomyces cerevisiae
-
 lcb1 and lcb2
-

Reaction

Reaction Comment Organism Reaction ID
palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2 mechanism Homo sapiens
a
palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2 mechanism Saccharomyces cerevisiae
a

Source Tissue

Source Tissue Comment Organism Textmining
carcinoma cell embryonic cell line PCC7-Mz1 Mus musculus
-
cell culture neuroblastoma cell line Homo sapiens
-
cell culture leukemia Molt-4 cells Homo sapiens
-
keratinocyte
-
 Homo sapiens
-
pancreas beta-cells Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + L-serine
-
 Mus musculus CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine
-
 Homo sapiens CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine
-
 Saccharomyces cerevisiae CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine involved in cellular stress response Homo sapiens CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine involved in cellular stress response Saccharomyces cerevisiae CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine first step of biosynthesis of sphingolipid bases Mus musculus CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine first step of biosynthesis of sphingolipid bases Homo sapiens CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine first step of biosynthesis of sphingolipid bases Saccharomyces cerevisiae CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine rate-limiting enzyme in synthesis of sphingolipids Homo sapiens CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine rate-limiting enzyme in synthesis of sphingolipids Saccharomyces cerevisiae CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine initial step of de novo ceramide biosynthesis Homo sapiens CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine initial step of de novo ceramide biosynthesis Saccharomyces cerevisiae CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Subunits

Subunits Comment Organism
More lcb1 and lcb2 are 2 subunits, both necessary for enzyme activity, complexed in ratio 1:1 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Mus musculus
pyridoxal 5'-phosphate required for activity Homo sapiens
pyridoxal 5'-phosphate required for activity Saccharomyces cerevisiae