Protein Variants | Comment | Organism |
---|---|---|
additional information | native gene contains a c.580delG frameshift mutation leading to expression of a truncated protein. Artificial reintroduction of residue G580 in the NAT3 gene leads to a functional enzyme able to acetylate several arylamine drugs | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0134 | - |
hydralazine | artificial full-length enzyme, pH 7.5, 37°C | Bacillus anthracis | |
0.0165 | - |
2-Aminofluorene | artificial full-length enzyme, pH 7.5, 37°C | Bacillus anthracis | |
0.189 | - |
4-Aminosalicylate | artificial full-length enzyme, pH 7.5, 37°C | Bacillus anthracis | |
0.349 | - |
isoniazid | artificial full-length enzyme, pH 7.5, 37°C | Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | A0A0J1HNH1 | isoform NAT3, native gene contains a c.580delG frameshift mutation leading to expression of an inactive truncated protein | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + 2-aminofluorene | - |
Bacillus anthracis | 4-nitrophenol + N-acetyl-2-aminofluorene | - |
? | |
4-nitrophenyl acetate + 4-aminosalicylate | - |
Bacillus anthracis | 4-nitrophenol + N-acetyl-4-aminosalicylate | - |
? | |
4-nitrophenyl acetate + hydralazine | - |
Bacillus anthracis | 4-nitrophenol + N-acetyl-hydralazine | - |
? | |
4-nitrophenyl acetate + isoniazid | - |
Bacillus anthracis | 4-nitrophenol + N-acetylisoniazid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(BACAN)NAT3 | - |
Bacillus anthracis |
ABW01_24350 | - |
Bacillus anthracis |
NAT3 | - |
Bacillus anthracis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
2-Aminofluorene | artificial full-length enzyme, pH 7.5, 37°C | Bacillus anthracis | |
0.51 | - |
isoniazid | artificial full-length enzyme, pH 7.5, 37°C | Bacillus anthracis | |
0.6 | - |
4-Aminosalicylate | artificial full-length enzyme, pH 7.5, 37°C | Bacillus anthracis | |
0.63 | - |
hydralazine | artificial full-length enzyme, pH 7.5, 37°C | Bacillus anthracis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
isoniazid | artificial full-length enzyme, pH 7.5, 37°C | Bacillus anthracis | |
3.3 | - |
4-Aminosalicylate | artificial full-length enzyme, pH 7.5, 37°C | Bacillus anthracis | |
26.1 | - |
2-Aminofluorene | artificial full-length enzyme, pH 7.5, 37°C | Bacillus anthracis | |
47 | - |
hydralazine | artificial full-length enzyme, pH 7.5, 37°C | Bacillus anthracis |