Protein Variants | Comment | Organism |
---|---|---|
Y190A | no significant alteration in the overall fold of the protein | Mesocricetus auratus |
Y190F | no significant alteration in the overall fold of the protein | Mesocricetus auratus |
Y190I | no significant alteration in the overall fold of the protein | Mesocricetus auratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
4-nitrophenyl acetate | mutant Y190A, pH 7.0, 25°C | Mesocricetus auratus | |
3.5 | - |
4-nitrophenyl acetate | mutant Y190F, pH 7.0, 25°C | Mesocricetus auratus | |
4.1 | - |
4-nitrophenyl acetate | mutant Y190I, pH 7.0, 25°C | Mesocricetus auratus | |
9 | - |
4-nitrophenyl acetate | wild-type, pH 7.0, 25°C | Mesocricetus auratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocricetus auratus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine | residue Y190 is critical for maximizing the acetylation rate of NAT2 and the transacetylation rate of substrate p-aminobenzoic acid. Y190 also plays an important role in determining the pKa of the active site Cys during acetylation, as well as the pH versus the rate profile for transacetylation | Mesocricetus auratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + 4-aminobenzoic acid | - |
Mesocricetus auratus | 4-nitrophenol + N-acetyl-4-aminobenzoic acid | - |
? | |
4-nitrophenyl acetate + anisidine | - |
Mesocricetus auratus | 4-nitrophenol + N-acetyl-anisidine | - |
? | |
4-nitrophenyl acetate + N-(4-aminobenzoyl)-L-glutamate | - |
Mesocricetus auratus | 4-nitrophenol + N-(4-acetylaminobenzoyl)-L-glutamate | - |
? | |
acetyl-CoA + 4-aminobenzoic acid | - |
Mesocricetus auratus | CoA + N-acetyl-4-aminobenzoic acid | - |
? | |
acetyl-CoA + 4-nitroaniline | - |
Mesocricetus auratus | CoA + N-acetyl-4-nitroaniline | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
4-nitroaniline | mutant Y190A, pH 7.0, 25°C | Mesocricetus auratus | |
0.31 | - |
4-nitroaniline | mutant Y190F, pH 7.0, 25°C | Mesocricetus auratus | |
0.6 | - |
4-nitroaniline | wild-type, pH 7.0, 25°C | Mesocricetus auratus | |
0.89 | - |
4-nitroaniline | mutant Y190I, pH 7.0, 25°C | Mesocricetus auratus | |
2 | 8 | anisidine | mutant Y190A, pH 7.0, 25°C | Mesocricetus auratus | |
8 | - |
4-Aminobenzoic acid | cosubstrate acetyl-CoA, mutant Y190A, pH 7.0, 25°C | Mesocricetus auratus | |
15 | - |
4-nitrophenyl acetate | mutant Y190A, pH 7.0, 25°C | Mesocricetus auratus | |
16 | - |
4-aminobenzoyl glutamic acid | mutant Y190A, pH 7.0, 25°C | Mesocricetus auratus | |
38 | - |
4-Aminobenzoic acid | cosubstrate 4-nitrophenyl acetate, mutant Y190A, pH 7.0, 25°C | Mesocricetus auratus | |
57 | - |
4-nitrophenyl acetate | mutant Y190I, pH 7.0, 25°C | Mesocricetus auratus | |
58 | - |
4-Aminobenzoic acid | cosubstrate acetyl-CoA, mutant Y190I, pH 7.0, 25°C | Mesocricetus auratus | |
60 | - |
4-Aminobenzoic acid | cosubstrate 4-nitrophenyl acetate, mutant Y190I, pH 7.0, 25°C | Mesocricetus auratus | |
77 | - |
4-aminobenzoyl glutamic acid | mutant Y190I, pH 7.0, 25°C | Mesocricetus auratus | |
85 | - |
anisidine | mutant Y190I, pH 7.0, 25°C | Mesocricetus auratus | |
86 | - |
4-aminobenzoyl glutamic acid | mutant Y190F, pH 7.0, 25°C | Mesocricetus auratus | |
120 | - |
4-aminobenzoyl glutamic acid | wild-type, pH 7.0, 25°C | Mesocricetus auratus | |
189 | - |
4-Aminobenzoic acid | cosubstrate acetyl-CoA, mutant Y190F, pH 7.0, 25°C | Mesocricetus auratus | |
200 | - |
4-Aminobenzoic acid | cosubstrate acetyl-CoA, wild-type, pH 7.0, 25°C | Mesocricetus auratus | |
260 | - |
anisidine | wild-type, pH 7.0, 25°C | Mesocricetus auratus | |
279 | - |
4-nitrophenyl acetate | mutant Y190F, pH 7.0, 25°C | Mesocricetus auratus | |
288 | - |
anisidine | mutant Y190F, pH 7.0, 25°C | Mesocricetus auratus | |
393 | - |
4-Aminobenzoic acid | cosubstrate 4-nitrophenyl acetate, mutant Y190F, pH 7.0, 25°C | Mesocricetus auratus | |
620 | - |
4-Aminobenzoic acid | cosubstrate 4-nitrophenyl acetate, wild-type, pH 7.0, 25°C | Mesocricetus auratus | |
1301 | - |
4-nitrophenyl acetate | wild-type, pH 7.0, 25°C | Mesocricetus auratus |