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Literature summary for 2.3.1.5 extracted from

  • Buranrat, B.; Prawan, A.; Sripa, B.; Kukongviriyapan, V.
    Inflammatory cytokines suppress arylamine N-acetyltransferase 1 in cholangiocarcinoma cells (2007), World J. Gastroenterol., 13, 6219-6225.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.5

Application

Application Comment Organism
medicine the findings show that inflammation can supress NAT1, a key cellular defense enzyme, such a suppression may be implicated in drug toxicity and cancer risk Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cytokine mixture of proinflammatory cytokines, interferon-gamma, interleukin-1beta, tumor necrosis factor-alpha Homo sapiens
proinflammatory cytokine treatment of cholangiocarcinoma KKU-100 cells with cytokines (interferon-gamma, interleukin-1beta and tumor necrosis factor-alpha) suppresses NAT1 activity, reducing the Vmax without affecting the Km Homo sapiens
S-nitroso-glutathione treatment of cholangiocarcinoma KKU-100 cells S-nitroso-glutathione results in reduced NAT1 activity as early as 2 h, and the suppression persists for 48 h Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 p-Aminobenzoic acid control Homo sapiens
0.0028
-
 p-Aminobenzoic acid using control cholangiocarcinoma KKU-100 cells without cytokine administration, Vmax: 211.2 pmol/min/mg Homo sapiens
0.0033
-
 p-Aminobenzoic acid cytokine mixture Homo sapiens
0.0033
-
 p-Aminobenzoic acid using control cholangiocarcinoma KKU-100 cells after treatment with the mixture of inflammatory cytokines for 48 h, Vmax: 135.7 pmol/min/mg. A significant decrease in Vmax is shown but without affecting Km, implying a change in the amount of enzyme but not in its affinity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cholangiocarcinoma cell cytokine treatment and S-nitroso-glutathione treatment of cholangiocarcinoma KKU-100 cells induces a decreased NAT1 mRNA expression Homo sapiens
-
KKU-100 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.054
-
 cytokine mixture, 2.5 microM p-aminobenzoic acid Homo sapiens
0.097
-
 cytokine mixture, 7.5 microM p-aminobenzoic acid Homo sapiens
0.108
-
 control, 2.5 microM p-aminobenzoic acid Homo sapiens
0.124
-
 cytokine mixture, 40 microM p-aminobenzoic acid Homo sapiens
0.146
-
 control, 7.5 microM p-aminobenzoic acid Homo sapiens
0.2
-
 control, 40 microM p-aminobenzoic acid Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + p-aminobenzoic acid
-
 Homo sapiens CoA + N-acetyl-4-aminobenzoic acid
-
 ?
acetyl-CoA + p-aminobenzoic acid activity assay Homo sapiens CoA + N-acetyl-4-aminobenzoic acid
-
 ?

Synonyms

Synonyms Comment Organism
arylamine N-acetyltransferase 1
-
 Homo sapiens
NAT1
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens
7.5
-
 activity assay Homo sapiens