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Literature summary for 2.3.1.5 extracted from

  • Wang, H.; Liu, L.; Hanna, P.E.; Wagner, C.R.
    Catalytic mechanism of hamster arylamine N-acetyltransferase 2 (2005), Biochemistry, 44, 11295-11306.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant NAT2 in Escherichia coli, the mutant variants are insoluble, inactive enzymes Mesocricetus auratus

Protein Variants

Protein Variants Comment Organism
H107N site-directed mutagenesis, no expression in Escherichia coli cells possible Mesocricetus auratus
H107Q site-directed mutagenesis, no expression in Escherichia coli cells possible, insoluble inactive enzyme Mesocricetus auratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics, kinetic and isotope effect studies Mesocricetus auratus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + an arylamine Mesocricetus auratus
-
CoA + an N-acetylarylamine
-
?
additional information Mesocricetus auratus the enzyme catalyzes an acetyl group transfer from AcCoA to primary arylamines, hydrazines, and hydrazides and play a very important role in the metabolism and bioactivation of drugs, carcinogens, and other xenobiotics ?
-
?

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus P50293 isozyme NAT2; isozyme NAT2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant NAT2 from Escherichia coli by anion exchange chromatography Mesocricetus auratus

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine ping pong reaction mechanism, the enzyme contains a strictly conserved a Cys-His-Asp catalytic triad, acetylation of the hamster NAT2 is dependent on a thiolate-imidazolium ion pair, Cys-S--His-ImH+, and not a general acid-base catalysis, a deacetylation mechanism dominated by nucleophilic attack of the thiol ester for arylamines Mesocricetus auratus

Renatured (Commentary)

Renatured (Comment) Organism
recovery of 25-50% activity of the recombinant wild-type enzyme after expression in Escherichia coli Mesocricetus auratus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Mesocricetus auratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + 4-aminobenzoic acid
-
Mesocricetus auratus 4-nitrophenol + N-acetyl-4-aminobenzoic acid
-
?
4-nitrophenyl acetate + 4-nitroaniline
-
Mesocricetus auratus 4-nitrophenol + N-acetyl-4-nitroaniline
-
?
4-nitrophenyl acetate + anisidine
-
Mesocricetus auratus 4-nitrophenol + N-acetyl-anisidine
-
?
4-nitrophenyl acetate + N-(4-aminobenzoyl)-L-glutamate
-
Mesocricetus auratus 4-nitrophenol + N-(4-acetylaminobenzoyl)-L-glutamate
-
?
acetyl-CoA + 4-aminobenzoic acid preferred substrate Mesocricetus auratus CoA + N-acetyl-4-aminobenzoic acid
-
?
acetyl-CoA + 4-aminobenzoyl-L-glutamic acid
-
Mesocricetus auratus CoA + 4-acetylaminobenzoyl-L-glutamic acid
-
?
acetyl-CoA + 4-nitroaniline
-
Mesocricetus auratus CoA + N-acetyl-4-nitroaniline
-
?
acetyl-CoA + an arylamine
-
Mesocricetus auratus CoA + an N-acetylarylamine
-
?
acetyl-CoA + anisidine
-
Mesocricetus auratus CoA + N-acetyl-anisidine
-
?
additional information the enzyme catalyzes an acetyl group transfer from AcCoA to primary arylamines, hydrazines, and hydrazides and play a very important role in the metabolism and bioactivation of drugs, carcinogens, and other xenobiotics Mesocricetus auratus ?
-
?
additional information 4-aminobenzoic acid is the preferred substrate Mesocricetus auratus ?
-
?

Synonyms

Synonyms Comment Organism
arylamine N-acetyltransferase 2
-
Mesocricetus auratus
NAT
-
Mesocricetus auratus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mesocricetus auratus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
4-nitroaniline pH 7.0, 25°C, with cofactor acetyl-CoA, recombinant wild-type enzyme Mesocricetus auratus
76
-
4-aminobenzoyl glutamic acid pH 7.0, 25°C, with cofactor acetyl-CoA, recombinant wild-type enzyme Mesocricetus auratus
120
-
4-aminobenzoyl glutamic acid pH 7.0, 25°C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme Mesocricetus auratus
200
-
4-Aminobenzoic acid pH 7.0, 25°C, with cofactor acetyl-CoA, recombinant wild-type enzyme Mesocricetus auratus
260
-
anisidine pH 7.0, 25°C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme Mesocricetus auratus
620
-
4-Aminobenzoic acid pH 7.0, 25°C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme Mesocricetus auratus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Mesocricetus auratus

pH Range

pH Minimum pH Maximum Comment Organism
5.2 9 pH profiles with cofactors acetyl-CoA and 4-nitrophenyl acetate, overview Mesocricetus auratus

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Mesocricetus auratus