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Literature summary for 2.3.1.5 extracted from

  • Westwood, I.M.; Holton, S.J.; Rodrigues-Lima, F.; Dupret, J.M.; Bhakta, S.; Noble, M.E.; Sim, E.
    Expression, purification, characterization and structure of Pseudomonas aeruginosa arylamine N-acetyltransferase (2005), Biochem. J., 385, 605-612.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression as an N-terminal histidine-tagged protein in Escherichia coli Pseudomonas aeruginosa
expression in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pseudomonas aeruginosa
hanging- and sitting-drop vapour diffusion method Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.136
-
 p-anisidine pH8, 37°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa
nickel ion affinity chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no detectable activity: sulphacetamide, sulphanilamide, sulphadiazine, sulphamethazine, trimethoprim, phenylhydrazine Pseudomonas aeruginosa
0.532
-
 4-aminobenzoic acid L-glutamate, pH 8, 37°C Pseudomonas aeruginosa
0.629
-
 aniline, pH 8, 37°C Pseudomonas aeruginosa
0.641
-
 4-methoxyphenylhydrazine, pH 8, 37°C Pseudomonas aeruginosa
1
-
 sulphisoxazole, pH 8, 37°C Pseudomonas aeruginosa
1.31
-
 sulphametoxazole, pH 8, 37°C Pseudomonas aeruginosa
2.324
-
 isoniazid, pH 8, 37°C Pseudomonas aeruginosa
2.76
-
 4-trifluoromethylaniline, pH 8, 37°C Pseudomonas aeruginosa
4.14
-
 4-chloroaniline, pH 8, 37°C Pseudomonas aeruginosa
5.71
-
 4-bromoaniline, pH 8, 37°C Pseudomonas aeruginosa
6.76
-
 3,4-dichloroaniline, pH 8, 37°C Pseudomonas aeruginosa
8.2
-
 4-aminobenzoic acid, pH 8, 37°C Pseudomonas aeruginosa
12.05
-
 4-hexyloxyaniline, pH 8, 37°C Pseudomonas aeruginosa
13.47
-
 4-anisidine, pH 8, 37°C Pseudomonas aeruginosa
13.8
-
 4-iodoaniline, pH 8, 37°C Pseudomonas aeruginosa
14
-
 4-chorobenzoic hydrazide, pH 8, 37°C Pseudomonas aeruginosa
18.66
-
 4-aminosalicylate, pH 8, 37°C Pseudomonas aeruginosa
19.24
-
 4-ethoxyaniline, pH 8, 37°C Pseudomonas aeruginosa
21.97
-
 4-phenoxyaniline, pH 8, 37°C Pseudomonas aeruginosa
25.66
-
 4-butoxyaniline, pH 8, 37°C Pseudomonas aeruginosa
29.55
-
 hydralazine, pH 8, 37°C Pseudomonas aeruginosa
44.51
-
 4-aminoveratrole, pH 8, 37°C Pseudomonas aeruginosa
44.71
-
 2-aminofluorene, pH 8, 37°C Pseudomonas aeruginosa
73.3
-
 5-aminosalicylate, pH 8, 37°C Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
4°C, 2 months, no apparent degradation of the enzyme, no change in activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2-aminofluorene
-
 Pseudomonas aeruginosa CoA + 2-acetylaminofluorene
-
 ?
acetyl-CoA + 2-aminofluorene
-
 Pseudomonas aeruginosa CoA + N-acetyl-2-aminofluorene
-
 ?
acetyl-CoA + 3,4-dichloraniline
-
 Pseudomonas aeruginosa CoA + N-acetyl-3,4-dichloraniline
-
 ?
acetyl-CoA + 3,4-dichloroaniline
-
 Pseudomonas aeruginosa CoA + N-acetyl-3,4-dichloroaniline
-
 ?
acetyl-CoA + 4-aminobenzoic acid
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-aminobenzoic acid
-
 ?
acetyl-CoA + 4-aminobenzoyl-L-glutamate
-
 Pseudomonas aeruginosa CoA + 4-acetylaminobenzoyl-L-glutamate
-
 ?
acetyl-CoA + 4-aminosalicylate
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-aminosalicylate
-
 ?
acetyl-CoA + 4-aminosalicylic acid
-
 Pseudomonas aeruginosa CoA + 4-acetylamino-2-hydroxybenzoate
-
 ?
acetyl-CoA + 4-aminoveratrole
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-aminoveratrole
-
 ?
acetyl-CoA + 4-aminoveratrole
-
 Pseudomonas aeruginosa CoA + 1,2-dimethoxy-4-acetylaminobenzene
-
 ?
acetyl-CoA + 4-anisidine
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-anisidine
-
 ?
acetyl-CoA + 4-anisidine
-
 Pseudomonas aeruginosa CoA + 4-methoxyacetanilide
-
 ?
acetyl-CoA + 4-bromaniline
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-bromaniline
-
 ?
acetyl-CoA + 4-bromoaniline
-
 Pseudomonas aeruginosa CoA + 4-bromoacetanilide
-
 ?
acetyl-CoA + 4-butoxyaniline
-
 Pseudomonas aeruginosa CoA + 4-butoxyacetanilide
-
 ?
acetyl-CoA + 4-butoxyaniline
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-butoxyaniline
-
 ?
acetyl-CoA + 4-chloroaniline
-
 Pseudomonas aeruginosa CoA + 4-chloroacetanilide
-
 ?
acetyl-CoA + 4-chloroaniline
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-chloroaniline
-
 ?
acetyl-CoA + 4-chlorobenzoic hydrazide
-
 Pseudomonas aeruginosa CoA + ?
-
 ?
acetyl-CoA + 4-chlorobenzoic hydrazide
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-chlorobenzoic hydrazide
-
 ?
acetyl-CoA + 4-ethoxyaniline
-
 Pseudomonas aeruginosa CoA + 4-ethoxyacetanilide
-
 ?
acetyl-CoA + 4-ethoxyaniline
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-ethoxyaniline
-
 ?
acetyl-CoA + 4-hexyloxyaniline
-
 Pseudomonas aeruginosa CoA + 4-hexyloxyacetanilide
-
 ?
acetyl-CoA + 4-hexyloxyaniline
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-hexyloxyaniline
-
 ?
acetyl-CoA + 4-iodoaniline
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-iodoaniline
-
 ?
acetyl-CoA + 4-iodoaniline
-
 Pseudomonas aeruginosa CoA + 4-iodoacetanilide
-
 ?
acetyl-CoA + 4-methoxyphenylhydrazine
-
 Pseudomonas aeruginosa CoA + ?
-
 ?
acetyl-CoA + 4-methoxyphenylhydrazine
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-methoxyphenylhydrazine
-
 ?
acetyl-CoA + 4-phenoxyaniline
-
 Pseudomonas aeruginosa CoA + 4-phenoxyacetanilide
-
 ?
acetyl-CoA + 4-phenoxyaniline
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-phenoxyaniline
-
 ?
acetyl-CoA + 4-trifluoromethylaniline
-
 Pseudomonas aeruginosa CoA + N-acetyl-4-trifluoromethylaniline
-
 ?
acetyl-CoA + 5-aminosalicylate
-
 Pseudomonas aeruginosa CoA + N-acetyl-5-aminosalicylate
-
 ?
acetyl-CoA + 5-aminosalicylic acid
-
 Pseudomonas aeruginosa CoA + 5-acetylamino-2-hydroxybenzoate
-
 ?
acetyl-CoA + aniline
-
 Pseudomonas aeruginosa CoA + N-acetylaniline
-
 ?
acetyl-CoA + aniline
-
 Pseudomonas aeruginosa CoA + acetanilide
-
 ?
acetyl-CoA + hydralazine
-
 Pseudomonas aeruginosa CoA + ?
-
 ?
acetyl-CoA + hydralazine
-
 Pseudomonas aeruginosa CoA + N-acetylhydralazine
-
 ?
acetyl-CoA + isoniazid
-
 Pseudomonas aeruginosa CoA + N-acetylisoniazid
-
 ?
acetyl-CoA + isoniazide
-
 Pseudomonas aeruginosa CoA + acetylniazide
-
 ?
acetyl-CoA + N-(4-acetylaminobenzoyl)-L-glutamate
-
 Pseudomonas aeruginosa CoA + 4-acetylaminobenzoyl-L-glutamate
-
 ?
acetyl-CoA + N-(4-aminobenzoyl)-L-glutamate
-
 Pseudomonas aeruginosa CoA + N-(4-acetylaminobenzoyl)-L-glutamate
-
 ?
acetyl-CoA + sulfamethoxazole
-
 Pseudomonas aeruginosa CoA + ?
-
 ?
acetyl-CoA + sulphamethoxazole
-
 Pseudomonas aeruginosa CoA + N-acetylsulphamethoxazole
-
 ?
acetyl-CoA + sulphisoxazole
-
 Pseudomonas aeruginosa CoA + 4-acetylamino-N-(3,4-dimethylisoxazol-5-yl)-benzenesulfonamide
-
 ?
acetyl-CoA + sulphisoxazole
-
 Pseudomonas aeruginosa CoA + N-acetylsulphisoxazole
-
 ?

Synonyms

Synonyms Comment Organism
PANAT
-
 Pseudomonas aeruginosa