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Literature summary for 2.3.1.5 extracted from

  • Dupret, J.M.; Grant, D.M.
    Site-directed mutagenesis of recombinant human arylamine N-acetyltransferase expressed in Escherichia coli. Evidence for direct involvement of Cys68 in the catalytic mechanism of polymorphic human NAT2 (1992), J. Biol. Chem., 267, 7381-7385.
    View publication on PubMed
Search for more literature for 2.3.1.5

Cloned(Commentary)

Cloned (Comment) Organism
NAT1 and NAT2, expressed in Escherichia coli XA90 Homo sapiens

Protein Variants

Protein Variants Comment Organism
C223G NAT2, enzymatically active, markedly reduced in vitro stability Homo sapiens
C44G NAT2, enzymatically active, markedly reduced in vitro stability Homo sapiens
C68G NAT2, no enzymatic activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5
-
 sulfamethazine isozyme NAT1 Homo sapiens
45
-
 procainamide isozyme NAT1 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2-aminofluorene N-acetyltransferase 2 Homo sapiens CoA + 2-acetylaminofluorene
-
 ?
acetyl-CoA + 4-aminosalicylic acid
-
 Homo sapiens CoA + 4-acetylamino-2-hydroxybenzoate
-
 ?
acetyl-CoA + procainamide N-acetyltransferase 2 Homo sapiens CoA + N-acetylprocainamide
-
 ?
acetyl-CoA + sulfamethazine N-acetyltransferase 2 Homo sapiens CoA + N-4-acetylsulfamethazine
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 t1/2: 35 h NAT2 wild-type, 3.5 h Cys44Gly-mutant, 0.5 h Cys223Gly-mutant Homo sapiens