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Literature summary for 2.3.1.5 extracted from

  • Hasmann, M.J.; Seidl, P.H.; Engelhardt, G.; Schleifer, K.H.
    Acetyl-coenzyme A: arylamine N-acetyltransferases in microorganisms: screening and isolation of an enzyme from Bacillus cereus (1986), Arch. Microbiol., 146, 275-279.
    View publication on PubMed
Search for more literature for 2.3.1.5

General Stability

General Stability Organism
dithiothreitol stabilizes Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 4-amino-4'-nitroazobenzene
-
 Bacillus cereus
0.28 0.37 4-nitroaniline
-
 Bacillus cereus
0.3
-
 4-nitrobenzalhydrazone
-
 Bacillus cereus
0.5
-
 3,5-dimethyl-4-nitroaniline
-
 Bacillus cereus
0.77
-
 acetyl-CoA
-
 Bacillus cereus
0.77 0.8 decylamine
-
 Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + an arylamine Bacillus cereus constitutive enzyme CoA + a N-acetylarylamine
-
 ?
acetyl-CoA + an arylamine Cordyceps militaris constitutive enzyme CoA + a N-acetylarylamine
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
 distribution among soil microorganisms, overview
-
Cordyceps militaris
-
 insect pathogen fungus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 3,5-dimethyl-4-nitroaniline
-
 Bacillus cereus CoA + ?
-
 ?
acetyl-CoA + 4-amino-4'-nitroazobenzene
-
 Bacillus cereus CoA + ?
-
 ?
acetyl-CoA + 4-nitroaniline
-
 Bacillus cereus CoA + N-acetyl-4-nitroaniline
-
 ?
acetyl-CoA + 4-nitroaniline
-
 Cordyceps militaris CoA + N-acetyl-4-nitroaniline
-
 ?
acetyl-CoA + 4-nitrobenzalhydrazone
-
 Bacillus cereus CoA + ?
-
 ?
acetyl-CoA + an arylamine constitutive enzyme Bacillus cereus CoA + a N-acetylarylamine
-
 ?
acetyl-CoA + an arylamine constitutive enzyme Cordyceps militaris CoA + a N-acetylarylamine
-
 ?
acetyl-CoA + aniline
-
 Bacillus cereus CoA + acetanilide
-
 ?
acetyl-CoA + aniline
-
 Cordyceps militaris CoA + acetanilide
-
 ?
acetyl-CoA + decylamine
-
 Bacillus cereus CoA + N-acetyldecylamine
-
 ?
additional information
-
 Cordyceps militaris ?
-
 ?
additional information no substrate: o-nitroaniline Bacillus cereus ?
-
 ?
additional information no substrate: 2-methyl-4-nitroaniline, 2,4-dinitroaniline, 4-nitrophenylhydrazine, carbopyridine Bacillus cereus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
-
 Bacillus cereus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
16 36 about half-maximal activity at 16°C and 36°C Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
34
-
 inactivation above Bacillus cereus

pH Range

pH Minimum pH Maximum Comment Organism
6.2 7.3 about half-maximal activity at pH 6.2 and 7.3 Bacillus cereus