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Literature summary for 2.3.1.48 extracted from

  • Lu Vo, T.; Park, J.; Seo, J.; Lee, E.; Choi, H.; Bae, S.; Le, H.; An, S.; Lee, H.; Wee, H.; Kim, K.
    ARD1-mediated aurora kinase A acetylation promotes cell proliferation and migration (2017), Oncotarget, 8, 57216-57230 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.48

Cloned(Commentary)

Cloned (Comment) Organism
gene ARD1, recombinant expression in HEK-293 cells, stable recombinant overexpression of GFP-tagged enzyme in MEF cells, recombinant expression of His-tagged enzyme and of GST-tagged enzyme in Escherichia coli strain BL21 Homo sapiens

Protein Variants

Protein Variants Comment Organism
R82A/Y122F site-directed mutagenesis, the enzyme mutant shows highly reduced lysine acetyltransferase activity with aurora kinase A as substrate Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
centrosome
-
 Homo sapiens 5813
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + [AuA]-L-lysine125 Homo sapiens lysine residues at positions 75 and 125 of aurora kinase A (AuA) are acetylated by ARD1, mutational analysis with AUA mutant substrates, overview CoA + [AuA]-N6-acetyl-L-lysine125
-
 ?
acetyl-CoA + [AuA]-L-lysine75 Homo sapiens lysine residues at positions 75 and 125 of aurora kinase A (AuA) are acetylated by ARD1, mutational analysis with AUA mutant substrates, overview CoA + [AuA]-N6-acetyl-L-lysine75
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P41227
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme and GST-tagged enzyme from Escherichia coli strain BL21 by nickel and glutathione affinity chromatography, respectively Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
additional information aurora kinase A colocalalizes with ARD1 during cell division and cell migration, aurora A colocalizes with ARD1 at centrosomes during cell division in prophase, metaphase, anaphase, and telophase Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + [AuA]-L-lysine125 lysine residues at positions 75 and 125 of aurora kinase A (AuA) are acetylated by ARD1, mutational analysis with AUA mutant substrates, overview Homo sapiens CoA + [AuA]-N6-acetyl-L-lysine125
-
 ?
acetyl-CoA + [AuA]-L-lysine75 lysine residues at positions 75 and 125 of aurora kinase A (AuA) are acetylated by ARD1, mutational analysis with AUA mutant substrates, overview Homo sapiens CoA + [AuA]-N6-acetyl-L-lysine75
-
 ?

Synonyms

Synonyms Comment Organism
ARD1 acetyltransferase
-
 Homo sapiens
arrest defective protein 1 acetyltransferase
-
 Homo sapiens
More see also EC 2.3.1.255 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction the AuA acetylation level decreases dramatically when ARD1 is mutated at R82 and Y122. The phosphorylation level of AuA is decreased in cells overexpressing K75R/K125R mutant Homo sapiens
metabolism aurora kinase A colocalalizes with ARD1 during cell division and cell migration Homo sapiens
physiological function ARD1-mediated aurora kinase A (AuA) acetylation promotes cell proliferation and migration. ARD1-mediated AuA acetylation at K75/K125 enhances AuA kinase activity. Cells overexpressing AuA double mutant K75R/K125R display a dramatically decreased level of acetylated AuA, suggesting that these sites are critical for the acetylation of AuA by ARD1. AuA interacts with ARD1, and AuA acetylation is regulated by functional ARD1 Homo sapiens