Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.48 extracted from

  • Qian, W.; Miki, D.; Zhang, H.; Liu, Y.; Zhang, X.; Tang, K.; Kan, Y.; La, H.; Li, X.; Li, S.; Zhu, X.; Shi, X.; Zhang, K.; Pontes, O.; Chen, X.; Liu, R.; Gong, Z.; Zhu, J.
    A histone acetyltransferase regulates active DNA demethylation in Arabidopsis (2012), Science, 336, 1445-1448.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in insect cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C732W mutation abolishes the binding to histone H3 Arabidopsis thaliana
C740W mutation abolishes the binding to histone H3 Arabidopsis thaliana
D924A mutation in conserved residues in the histone acetyltransferase domain, abolishes the in vitro acetyltransferase activity Arabidopsis thaliana
E451Stop mutation results in the silencing of 35S-NPTII but not the RD29A-LUC transgene Arabidopsis thaliana
E941Q mutation in conserved residues in the histone acetyltransferase domain, abolishes the in vitro acetyltransferase activity Arabidopsis thaliana
M942A mutation in conserved residues in the histone acetyltransferase domain, diminishes the in vitro acetyltransferase activity Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana F4IXE7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + histone H3
-
Arabidopsis thaliana CoA + acetylhistone H3 residues K14, K18, and K23 of H3 are acetylated by domain C1 of isoform Idm1 in vitro ?

Subunits

Subunits Comment Organism
More isoform IDM1 is predicted to have a metal-binding domain, a plant homeo finger domain and an N-acetyltransferase domain. The plant homeo finger of IDM1 specifically binds the N-terminal tail of histone H3 and the binding is inhibited by H3K4 di- or trimethylation Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
At3g14980
-
Arabidopsis thaliana
Idm1
-
Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function isoform IDM1is a regulator of DNA demethylation. IDM1 is required for preventing DNA hypermethylation of highly homologous multicopy genes and other repetitive sequences that are normally targeted for active DNA demethylation by Repressor of Silencing 1 and related 5-methylcytosine DNA glycosylases. IDM1 binds methylated DNA at chromatin sites lacking histone H3K4 di- or trimethylation and acetylates H3 to create a chromatin environment permissible for 5-methylcytosine DNA glycosylases to function Arabidopsis thaliana