Cloned (Comment) | Organism |
---|---|
expression of His6-tagged wild-type and mutant Esa1s in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C304S | site-directed mutagenesis, the mutant shows 97.4% reduced histone H4 acetylation at pH 8.0 compared to the wild-type Esa1 | Saccharomyces cerevisiae |
C304S/E338Q | site-directed mutagenesis, the mutant protein is intrinsically unstable and catalytically inactive, the mutant cell is lethal | Saccharomyces cerevisiae |
D343V | site-directed mutagenesis, the mutant shows reduced histone H4 acetylation compared to the wild-type Esa1 | Saccharomyces cerevisiae |
E338Q | site-directed mutagenesis, the mutant shows 99.5% reduced histone H4 acetylation at pH 8.0 compared to the wild-type Esa1, recombinant Esa1-E338Q displays detectable HATactivity at elevated pH of 9.2 | Saccharomyces cerevisiae |
L357H | site-directed mutagenesis, the mutant shows 41% reduced histone H4 acetylation compared to the wild-type Esa1 | Saccharomyces cerevisiae |
W66R | site-directed mutagenesis, the mutant shows reduced histone H4 acetylation compared to the wild-type Esa1 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | Esa1 is the catalytic subunit of at least two multiprotein complexes, NuA4 and Piccolo NuA4, picNuA4 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
diverse strains | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + histone H3 | - |
Saccharomyces cerevisiae | CoA + acetylhistone H3 | - |
? | |
additional information | Esa1 is the catalytic subunit of at least two multiprotein complexes, NuA4 and Piccolo NuA4, picNuA4 | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Esa1 | - |
Saccharomyces cerevisiae |
HAT | - |
Saccharomyces cerevisiae |
More | the enzyme belongs to the MYST acetyltransferase family | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9.2 | assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | Esa1 catalytic HAT activity is essential in yeast binding acetyl-CoA or lysine substrates and positively regulating the activities of NuA4 and Piccolo NuA4, Esa1 is involved in DNA damage repair | Saccharomyces cerevisiae |