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Literature summary for 2.3.1.48 extracted from

  • Toleman, C.A.; Paterson, A.J.; Kudlow, J.E.
    The histone acetyltransferase NCOAT contains a zinc finger-like motif involved in substrate recognition (2006), J. Biol. Chem., 281, 3918-3925.
    View publication on PubMed

Application

Application Comment Organism
molecular biology the catalytically inactive mutant Y891F is useful for stable binding and purification of unacetylated histone H4 protein Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged enzyme in BSC40 cells via VTF-7 vaccinia virus transfection vector Mus musculus

Protein Variants

Protein Variants Comment Organism
Y891F site-directed mutagenesis, catalytically inactive mutant, stable binding of unacetylated histone H4, useful for purification of the histone protein Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleoplasm
-
Mus musculus 5654
-
nucleosome
-
Mus musculus 786
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not bind Zn2+ Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
histone H4 + acetyl-CoA Mus musculus
-
acetyl-histone H4 + CoA
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from BSC40 cells by glutathione affinity chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
histone H4 + acetyl-CoA
-
Mus musculus acetyl-histone H4 + CoA
-
?
histone H4 + acetyl-CoA acetylation at Lys8, NCOAT has the ability to directly associate with both an acetylated and unacetylated histone H4 tail in vitro without tequiring acetyl-lysine contacts, binding and interaction mechanism, overview Mus musculus acetyl-histone H4 + CoA
-
?
additional information the nuclear cytoplasmic O-GlcNAcase and acetyltransferase, NCOAT, is a bifunctional enzyme with both glycoside hydrolase and alkyltransferase activity and contains a zinc finger-like motif responsible for substrate recognition, via making contacts with the histone tails within nucleosomes, essential for activity, overview Mus musculus ?
-
?

Subunits

Subunits Comment Organism
More NCOAT contains a motif similar to the histone binding domain in the MYST family of HATs, a zinc finger-like motif responsible for substrate recognition Mus musculus

Synonyms

Synonyms Comment Organism
HAT
-
Mus musculus
More the enzyme belongs to the MYST family of histone acetyltransferases Mus musculus
NCOAT
-
Mus musculus
nuclear cytoplasmicO-GlcNAcase and acetyltransferase
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Mus musculus