Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.48 extracted from

  • Tanner, K.G.; Langer, M.R.; Kim, Y.; Denu, J.M.
    Kinetic mechanism of the histone acetyltransferase GCN5 from yeast (2000), J. Biol. Chem., 275, 22048-22055.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of Gcn5 protein catalytic domain in Escherichia coli BL21 (DE3) Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
acetylated histone H3 peptide noncompetitive versus acetyl-CoA and histone H3 Saccharomyces cerevisiae
CoA competitive versus acetyl-CoA, Gnc5 protein Saccharomyces cerevisiae
desulfo-coenzyme A dead-end inhibitor, competitive versus acetyl-CoA, Gcn5 protein Saccharomyces cerevisiae
etheno-CoA
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
acetyl-CoA Gcn5 protein Saccharomyces cerevisiae
0.49
-
histone H3-peptide Gcn5 protein Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl 7fold decrease in activity between 0.15 and 1 M Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
gene Gcn5
-

Purification (Commentary)

Purification (Comment) Organism
recombinant catalytic domain of Gcn5 protein Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine ternary complexed mechanism for PCAF and Gnc5 Saccharomyces cerevisiae
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine PCAF and Gcn5 protein, fully ordered Bi-Bi kinetic mechanism with acetyl-CoA binding before histone H3 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + histone
-
Saccharomyces cerevisiae CoA + acetylhistone
-
r
acetyl-CoA + histone H3
-
Saccharomyces cerevisiae CoA + acetylhistone H3
-
?
acetyl-CoA + histone H3 peptide Lys14 of histone H3 and a peptide containing Lys14 thereof are the preferred substrates for Gnc5 and PCAF protein, as well as Gnc5 and PCAF catalytic domain Saccharomyces cerevisiae CoA + acetylhistone H3 peptide
-
?
acetyl-CoA + histone H3-peptide
-
Saccharomyces cerevisiae CoA + acetylhistone H3-peptide
-
?
acetyl-CoA + histone H3-peptide
-
Saccharomyces cerevisiae CoA + acetylhistone H3 -peptide
-
?
additional information Gcn5 and PCAF protein are transcription cofactors Saccharomyces cerevisiae ?
-
?

Synonyms

Synonyms Comment Organism
HAT
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0283
-
acetyl-CoA Gcn5 protein Saccharomyces cerevisiae
0.0283
-
histone H3-peptide Gcn5 protein Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Saccharomyces cerevisiae