Cloned (Comment) | Organism |
---|---|
expression of the catalytic domain of PCAF protein, amino acid residues 493-658, in Escherichia coli BL21 (DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetylated histone H3 peptide | acetylated at Lys14, product inhibition of PCAF protein, noncompetitive against both substrates | Homo sapiens | |
CoA | product inhibition of PCAF protein, competitive against acetyl-CoA | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
PCAF protein | - |
Purification (Comment) | Organism |
---|---|
recombinant catalytic domain of PCAF protein from E. coli | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine | PCAF and Gcn5 protein, fully ordered Bi-Bi kinetic mechanism with acetyl-CoA binding before histone H3 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + histone | - |
Homo sapiens | CoA + acetylhistone | - |
? | |
acetyl-CoA + histone H3 | Lys14 of histone H3 and a peptide containing Lys14 thereof are the preferred substrates for Gnc5 and PCAF protein, as well as Gnc5 and PCAF catalytic domain | Homo sapiens | CoA + acetylhistone H3 | - |
? | |
acetyl-CoA + histone H3 peptide | Lys14 of histone H3 and a peptide containing Lys14 thereof are the preferred substrates for Gnc5 and PCAF protein, as well as Gnc5 and PCAF catalytic domain | Homo sapiens | CoA + acetylhistone H3 peptide | - |
? | |
acetyl-CoA + histone H3-peptide | - |
Homo sapiens | CoA + acetylhistone H3 -peptide | - |
? | |
additional information | Gcn5 and PCAF protein are transcription cofactors | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HAT | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0383 | - |
acetyl-CoA | PCAF catalytic domain | Homo sapiens | |
0.0383 | - |
histone H3-peptide | PCAF catalytic domain | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
reaction kinetic is pH-dependent | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | PCAF protein | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00044 | - |
coenzyme A | PCAF catalytic domain | Homo sapiens | |
0.00066 | - |
acetylated histone H3 peptide | PCAF catalytic domain | Homo sapiens |