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Literature summary for 2.3.1.47 extracted from
- Biotin synthesis in plants. The first committed step of the pathway is catalyzed by a cytosolic 7-keto-8-aminopelargonic acid synthase (2005), Plant Physiol., 139, 1666-1676.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bioF, DNA and amino acid sequence determination and analysis, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | functional complementation of an Escherichia coli mutant deficient in KAPA synthase reaction | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the recombinant KAPA synthase obeys Michaelis-Menten kinetics with respect to pimeloyl-CoA and L-Ala | Arabidopsis thaliana | |
| 0.0016 | - |
pimeloyl-CoA | pH 7.5, 30°C, recombinant enzyme | Arabidopsis thaliana |  |
| 1.4 | - |
L-alanine | pH 7.5, 30°C, recombinant enzyme | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Arabidopsis thaliana | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
recombinant His-tagged enzyme, gel filtration | Arabidopsis thaliana |
| 52171 | - |
1 * 52171, amino acid sequence calculation | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Arabidopsis thaliana | the enzyme catalyzes the first committed enzyme of the biotin synthesis pathway | ? | - |
? | |
| pimeloyl-CoA + L-alanine | Arabidopsis thaliana | - |
8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q8GW43 | ecotype Wassilewskija | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme partially by subcellular fractionation, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Arabidopsis thaliana |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | via an AtbioF-PLP internal aldimine complex, i.e. a Schiff base formed between PLP and a Lys residue in the protein | Arabidopsis thaliana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.09 | 0.1 | purified recombinant enzyme | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme catalyzes the first committed enzyme of the biotin synthesis pathway | Arabidopsis thaliana | ? | - |
? | |
| pimeloyl-CoA + L-alanine | - |
Arabidopsis thaliana | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
| pimeloyl-CoA + L-alanine | the enzyme is strictly stereospecific for L-alanine | Arabidopsis thaliana | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 52171, amino acid sequence calculation | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 7-keto-8-aminopelargonic acid synthase | - |
Arabidopsis thaliana |
| AtbioF | - |
Arabidopsis thaliana |
| KAPA synthase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
pimeloyl-CoA | pH 7.5, 30°C, recombinant enzyme | Arabidopsis thaliana | |
| 0.11 | - |
L-alanine | pH 7.5, 30°C, recombinant enzyme | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | dependent on | Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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