Crystallization (Comment) | Organism |
---|---|
enzyme in complex with inhibitor trifluoroalanine, soaking of holo-enzyme crystals in a solution containing 10 mM L-trifluoroalanine and 10 mM pyridoxal-5'-phosphate in crystallization buffer containing 1.6 M ammonium sulfate and 200 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane at pH 8.5, and rapidly frozen in liquid nitrogen, X-ray diffraction structure determination and analysis at 1.99 A resolution | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
trifluoroalanine | binding structure, irreversible suicide inhibition of the enzyme, involves decarboxylative defluorination of the inhibitor-PLP aldimine followed by attack of the conjugated imine by the amino group of the active site lysine to afford a covalently bound difluorinated intermediate, which can subsequently undergo further HF losses and hydrolysis to afford a 2-(pyridoximine phosphate) acetoyl protein adduct | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41690 | - |
1 * 41690, native enzyme with bound cofactor, mass spectrometry | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxyhexanoyl-CoA + L-alanine | Escherichia coli | - |
8-amino-7-oxononanoate + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | detailed catalytic reaction mechanism, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxyhexanoyl-CoA + L-alanine | - |
Escherichia coli | 8-amino-7-oxononanoate + CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41690, native enzyme with bound cofactor, mass spectrometry | Escherichia coli |
More | comparison of the AONS active site in the apo-enzyme, the holo-enzyme, the difluoro-adduct, and the product aldimine, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
8-amino-7-oxononanoate synthase | - |
Escherichia coli |
AONS | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on, binding structure | Escherichia coli |