Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.47 extracted from

  • Alexeev, D.; Baxter, R.L.; Campopiano, D.J.; Kerbarh, O.; Sawyer, L.; Tomczyk, N.; Watt, R.; Webster, S.P.
    Suicide inhibition of alpha-oxamine synthases: structures of the covalent adducts of 8-amino-7-oxononanoate synthase with trifluoroalanine (2006), Org. Biomol. Chem., 4, 1209-1212.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with inhibitor trifluoroalanine, soaking of holo-enzyme crystals in a solution containing 10 mM L-trifluoroalanine and 10 mM pyridoxal-5'-phosphate in crystallization buffer containing 1.6 M ammonium sulfate and 200 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane at pH 8.5, and rapidly frozen in liquid nitrogen, X-ray diffraction structure determination and analysis at 1.99 A resolution Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
trifluoroalanine binding structure, irreversible suicide inhibition of the enzyme, involves decarboxylative defluorination of the inhibitor-PLP aldimine followed by attack of the conjugated imine by the amino group of the active site lysine to afford a covalently bound difluorinated intermediate, which can subsequently undergo further HF losses and hydrolysis to afford a 2-(pyridoximine phosphate) acetoyl protein adduct Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41690
-
1 * 41690, native enzyme with bound cofactor, mass spectrometry Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-carboxyhexanoyl-CoA + L-alanine Escherichia coli
-
8-amino-7-oxononanoate + CoA + CO2
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] detailed catalytic reaction mechanism, overview Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-carboxyhexanoyl-CoA + L-alanine
-
Escherichia coli 8-amino-7-oxononanoate + CoA + CO2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 41690, native enzyme with bound cofactor, mass spectrometry Escherichia coli
More comparison of the AONS active site in the apo-enzyme, the holo-enzyme, the difluoro-adduct, and the product aldimine, overview Escherichia coli

Synonyms

Synonyms Comment Organism
8-amino-7-oxononanoate synthase
-
Escherichia coli
AONS
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on, binding structure Escherichia coli