Literature summary for 2.3.1.46 extracted from

Born, T.L.; Blanchard, J.S.
Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Escherichia coli metA-encoded homoserine transsuccinylase (1999), Biochemistry, 38, 14416-14423.

Search for more literature for 2.3.1.46

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of metA gene in Escherichia coli BL21 (DE3)	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
iodoacetamide	inhibition is pH-dependent	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.17	-	succinyl-CoA	recombinant protein	Escherichia coli
0.18	-	glutaryl-CoA	recombinant protein	Escherichia coli
0.64	-	coenzyme A	recombinant protein	Escherichia coli
1.6	-	L-homoserine	recombinant protein	Escherichia coli
3.5	-	O-succinylhomoserine	recombinant protein	Escherichia coli
10	-	D-homoserine	recombinant protein	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35600	-	2 * 35600, SDS-PAGE	Escherichia coli
86000	-	gel filtration	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
succinyl-CoA + L-homoserine	Escherichia coli	involved in cystathionine and methionine biosynthesis	CoA + O-succinyl-L-homoserine	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	metA gene	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from E. coli BL21 (DE3)	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
succinyl-CoA + L-homoserine = CoA + O-succinyl-L-homoserine	ping-pong mechanism, enzyme contains a cysteine in the active site, member of the acyltransferase family	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutaryl-CoA + L-homoserine	-	Escherichia coli	CoA + O-glutaryl-L-homoserine	-	r
additional information	3-amino-1-propanol, L-serine, and L-threonine are poor substrates	Escherichia coli	?	-	?
additional information	no activity with acetyl-CoA, propionyl-CoA, butyryl-CoA, crotonyl-CoA, malonyl-CoA, and gamma-hydroxybutyric acid, and gamma-aminobutyric acid	Escherichia coli	?	-	?
succinyl-CoA + D-homoserine	-	Escherichia coli	CoA + O-succinyl-D-homoserine	-	r
succinyl-CoA + L-homoserine	-	Escherichia coli	CoA + O-succinyl-L-homoserine	-	r
succinyl-CoA + L-homoserine	involved in cystathionine and methionine biosynthesis	Escherichia coli	CoA + O-succinyl-L-homoserine	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 35600, SDS-PAGE	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.6	-	glutaryl-CoA	recombinant protein	Escherichia coli
5.23	-	coenzyme A	recombinant protein	Escherichia coli
5.23	-	O-succinyl-L-homoserine	recombinant protein	Escherichia coli
12	-	D-homoserine	recombinant protein	Escherichia coli
24	-	succinyl-CoA	recombinant protein	Escherichia coli
24	-	L-homoserine	recombinant protein	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	broad maximum	Escherichia coli

pH Range

pH Minimum	pH Maximum	Comment	Organism
additional information	-	pKs of 6.6 and about 7.9	Escherichia coli
6	8.7	-	Escherichia coli

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Release 2023.1 (January 2023)